Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 637787..638586 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag K5P75_RS03140 Protein ID WP_000347267.1
Coordinates 637787..638251 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K5P75_RS03145 Protein ID WP_001307405.1
Coordinates 638251..638586 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS03110 (632788) 632788..633222 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K5P75_RS03115 (633240) 633240..634118 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K5P75_RS03120 (634108) 634108..634887 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K5P75_RS03125 (634898) 634898..635371 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K5P75_RS03130 (635394) 635394..636674 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
K5P75_RS03135 (636923) 636923..637732 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K5P75_RS03140 (637787) 637787..638251 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K5P75_RS03145 (638251) 638251..638586 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K5P75_RS03150 (638735) 638735..640306 - 1572 WP_001273741.1 galactarate dehydratase -
K5P75_RS03155 (640681) 640681..642015 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K5P75_RS03160 (642031) 642031..642801 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T214113 WP_000347267.1 NZ_CP082120:c638251-637787 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T214113 NZ_CP109966:179061-179327 [Catenovulum sp. TS8]
ATGTATACTTTTGAATATGACCCTAATAAAAGTGAAATCAATCTCGTGAAACATGGTATCGATTTTGATGAAGCTCAAGA
GTTGTGGAATGATCCAGATTTAGTTGAGATCCCTGCAAATACATCAGATGAACCTCGTTCATTGATAATTGCATACCGCA
AAGGCAAGTTTTGGTCTGCTGTAATTACTCACCGAACTCCAAATATTCGGATAATTTCAGTTAGACGTTCTAGAAAAAAT
GAGGTTGAATTTTATGAAAGCTTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT214113 WP_001307405.1 NZ_CP082120:c638586-638251 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214113 NZ_CP109966:179314-179559 [Catenovulum sp. TS8]
ATGAAAGCTTCTGAATTTGAAAAAAAATTTGATGATGGTGACGATATTGTATCTAGCCTTGATCTTTCAAAGGCCAAGCG
CACGACACAGGAAACTAAGCGGGTTAATGTAGATTTTCCTACATGGATGCTTGATTCACTAGATCGTGAGGCTAATAGAG
TGGGTGTCACTAGACAGTCAATAATTAAAGTTTGGCTTGCTGAGCGTTTAGAGCATTTGCAAAGTCACGGATTTAAACAC
GGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References