Detailed information of TA system TA021411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 460759..461462
• Replicon: chromosome
• Accession: NC_007777
• Organism: Frankia sp. CcI3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q2JG16
• Locus tag: FRANCCI3_RS01925
• Protein ID: WP_011434854.1
• Coordinates: 460759..461151 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FRANCCI3_RS01930
• Protein ID: WP_011434855.1
• Coordinates: 461148..461462 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRANCCI3_RS25835	456088..456336	-	249	WP_162238919.1	hypothetical protein	-
FRANCCI3_RS01895	456233..458050	+	1818	WP_085949858.1	phosphomethylpyrimidine synthase ThiC	-
FRANCCI3_RS01900	458307..458636	+	330	WP_011434849.1	ABATE domain-containing protein	-
FRANCCI3_RS01905	458676..458921	+	246	WP_011434850.1	helix-turn-helix transcriptional regulator	-
FRANCCI3_RS26615	458918..459076	+	159	WP_023840839.1	hypothetical protein	-
FRANCCI3_RS01910	459519..459758	+	240	WP_011434851.1	DUF433 domain-containing protein	-
FRANCCI3_RS01915	459755..459958	+	204	Protein_392	DUF5615 family PIN-like protein	-
FRANCCI3_RS23095	460203..460688	+	486	WP_011434853.1	tyrosine-type recombinase/integrase	-
FRANCCI3_RS01925	460759..461151	+	393	WP_011434854.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FRANCCI3_RS01930	461148..461462	+	315	WP_011434855.1	helix-turn-helix transcriptional regulator	Antitoxin
FRANCCI3_RS01935	461909..463168	+	1260	Protein_396	transposase	-
FRANCCI3_RS01940	463192..464274	-	1083	WP_052154563.1	ISAs1 family transposase	-
FRANCCI3_RS01945	464725..466224	+	1500	WP_108913866.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	446983..479396	32413

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-116)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-87)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14560.64 Da
• Isoelectric Point: 10.2661

>T21411 WP_011434854.1 NC_007777:460759-461151 [Frankia casuarinae]
MAATPARWTIKATSEVREWLRSLRREEPDSYRSVNAAIDMLAEAGPALGRPLVDTLKGSTISNLKELRPRSGREVAIRVI
FVFDPWSQAVLLVAGNKAGDWSHWYRAAIPAAEVVYDGWLAVERKRREGS

Nucleotide

• Length: 393 bp

>T21411 NC_007777:460759-461151 [Frankia casuarinae]
ATGGCCGCAACGCCCGCCCGGTGGACGATCAAGGCCACCTCCGAGGTGCGAGAGTGGCTGCGTTCGCTGCGCCGGGAGGA
GCCGGACAGCTACCGATCCGTCAACGCGGCAATCGACATGCTCGCCGAGGCCGGGCCGGCGCTCGGGCGCCCGCTGGTCG
ACACCCTCAAGGGATCAACGATCAGCAACCTCAAGGAGCTCCGACCGCGGTCGGGCCGCGAGGTGGCCATCCGAGTGATC
TTCGTGTTCGATCCATGGTCGCAGGCCGTGCTGCTGGTCGCGGGGAACAAGGCCGGCGACTGGTCGCACTGGTACAGGGC
TGCGATTCCTGCGGCAGAGGTTGTCTACGACGGCTGGCTGGCCGTCGAACGGAAACGGCGGGAGGGCTCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.12 Da
• Isoelectric Point: 4.7324

>AT21411 WP_011434855.1 NC_007777:461148-461462 [Frankia casuarinae]
MTEFHDWEDIRAELHDGDDAALAVERARTEAWASAYHLAEERKRLGLTQRQVADLMGVSPGRISQIENGDLDVNEVATLS
RYARALGARMRIIFDYGNDLRQIA

Nucleotide

• Length: 315 bp

>AT21411 NC_007777:461148-461462 [Frankia casuarinae]
ATGACCGAGTTCCATGACTGGGAGGACATCCGCGCCGAGCTCCATGACGGCGATGACGCGGCACTCGCCGTGGAACGAGC
GCGTACGGAGGCGTGGGCCAGCGCCTACCACCTGGCCGAGGAGCGCAAGCGCCTTGGTTTGACACAGCGTCAGGTCGCCG
ATCTGATGGGCGTCTCACCTGGCCGGATAAGCCAGATCGAAAACGGTGATCTGGACGTGAACGAGGTGGCAACCCTGAGC
CGATACGCCCGGGCGTTGGGCGCACGCATGCGGATCATCTTCGACTACGGTAATGACCTCCGCCAGATCGCCTGA

Structures

Toxin

• AlphaFold DB: Q2JG16