Detailed information of TA system TA214104

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68160..68413
• Replicon: plasmid pKP19-2196-KPC2
• Accession: NZ_CP082042
• Organism: Klebsiella pneumoniae strain KP19-2196

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K6165_RS28000
• Protein ID: WP_001312851.1
• Coordinates: 68264..68413 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68160..68219 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6165_RS27960 (63820)	63820..63885	-	66	Protein_76	helix-turn-helix domain-containing protein	-
K6165_RS27965 (63938)	63938..64642	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6165_RS27970 (64667)	64667..64867	+	201	WP_072354025.1	hypothetical protein	-
K6165_RS27975 (64887)	64887..65633	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
K6165_RS27980 (65688)	65688..66248	+	561	WP_000139328.1	fertility inhibition protein FinO	-
K6165_RS27985 (66380)	66380..66580	+	201	WP_015059022.1	hypothetical protein	-
K6165_RS27990 (66966)	66966..67565	+	600	WP_032083981.1	PIN domain-containing protein	-
K6165_RS27995 (67627)	67627..67959	+	333	WP_152916585.1	hypothetical protein	-
- (68160)	68160..68219	-	60	NuclAT_1	-	Antitoxin
- (68160)	68160..68219	-	60	NuclAT_1	-	Antitoxin
- (68160)	68160..68219	-	60	NuclAT_1	-	Antitoxin
- (68160)	68160..68219	-	60	NuclAT_1	-	Antitoxin
K6165_RS28000 (68264)	68264..68413	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K6165_RS28005 (68697)	68697..68945	+	249	WP_000083837.1	replication regulatory protein RepA	-
K6165_RS30130 (69060)	69060..69238	+	179	Protein_86	protein CopA/IncA	-
K6165_RS28015 (69257)	69257..70114	+	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
K6165_RS28020 (71053)	71053..71706	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K6165_RS28025 (71799)	71799..72056	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K6165_RS28030 (71989)	71989..72390	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K6165_RS28035 (72639)	72639..73054	+	416	Protein_91	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B / blaSHV-12	-	1..160563	160563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T214104 WP_001312851.1 NZ_CP082042:68264-68413 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T214104 NZ_CP109963:c4618576-4618292 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT214104 NZ_CP082042:c68219-68160 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin