Detailed information of TA system TA214081

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89948..90374
• Replicon: plasmid pKP18-2172-KPC2
• Accession: NZ_CP082039
• Organism: Klebsiella pneumoniae strain KP18-2172

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6161_RS28135
• Protein ID: WP_001372321.1
• Coordinates: 89948..90073 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90150..90374 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6161_RS28100 (85003)	85003..85230	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
K6161_RS28105 (85324)	85324..86010	-	687	WP_015059009.1	PAS domain-containing protein	-
K6161_RS28110 (86201)	86201..86584	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6161_RS28115 (86861)	86861..87508	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K6161_RS28120 (87805)	87805..88626	-	822	WP_001234445.1	DUF932 domain-containing protein	-
K6161_RS28125 (88737)	88737..89033	-	297	WP_001272251.1	hypothetical protein	-
K6161_RS28130 (89333)	89333..89629	+	297	Protein_115	hypothetical protein	-
K6161_RS28135 (89948)	89948..90073	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6161_RS28810 (90015)	90015..90164	-	150	Protein_117	plasmid maintenance protein Mok	-
- (90150)	90150..90374	-	225	NuclAT_0	-	Antitoxin
- (90150)	90150..90374	-	225	NuclAT_0	-	Antitoxin
- (90150)	90150..90374	-	225	NuclAT_0	-	Antitoxin
- (90150)	90150..90374	-	225	NuclAT_0	-	Antitoxin
K6161_RS28140 (90386)	90386..91105	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
K6161_RS28145 (91102)	91102..91536	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K6161_RS28150 (91605)	91605..93628	-	2024	Protein_120	ParB/RepB/Spo0J family partition protein	-
K6161_RS28155 (93689)	93689..93922	-	234	WP_000006003.1	DUF905 family protein	-
K6161_RS28160 (93980)	93980..94507	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
K6161_RS28815 (94809)	94809..95276	+	468	Protein_123	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B / blaSHV-187	-	1..160747	160747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T214081 WP_001372321.1 NZ_CP082039:c90073-89948 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T214081 NZ_CP109953:3908556-3909083 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT214081 NZ_CP082039:c90374-90150 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin