Detailed information of TA system TA214051

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25640..25909
• Replicon: plasmid pKP18-2138-KPC2
• Accession: NZ_CP082034
• Organism: Klebsiella pneumoniae strain KP18-2138

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6163_RS28115
• Protein ID: WP_001372321.1
• Coordinates: 25784..25909 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25640..25705 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6163_RS28090	21350..21877	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
K6163_RS28095	21935..22168	+	234	WP_000006003.1	DUF905 family protein	-
K6163_RS28100	22229..24252	+	2024	Protein_30	ParB/RepB/Spo0J family partition protein	-
K6163_RS28105	24321..24755	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K6163_RS28110	24752..25471	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	25483..25707	+	225	NuclAT_0	-	-
-	25483..25707	+	225	NuclAT_0	-	-
-	25483..25707	+	225	NuclAT_0	-	-
-	25483..25707	+	225	NuclAT_0	-	-
-	25640..25705	-	66	-	-	Antitoxin
K6163_RS29015	25693..25842	+	150	Protein_33	plasmid maintenance protein Mok	-
K6163_RS28115	25784..25909	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6163_RS28120	26228..26524	-	297	Protein_35	hypothetical protein	-
K6163_RS28125	26824..27120	+	297	WP_001272251.1	hypothetical protein	-
K6163_RS28130	27231..28052	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K6163_RS28135	28349..28996	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K6163_RS28140	29273..29656	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6163_RS28145	29847..30533	+	687	WP_015059009.1	PAS domain-containing protein	-
K6163_RS28150	30627..30854	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaSHV-12 / blaKPC-2	-	1..115559	115559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T214051 WP_001372321.1 NZ_CP082034:25784-25909 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T214051 NZ_CP109948:356616-356963 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT214051 NZ_CP082034:c25705-25640 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin