214020

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4067041..4067638 Replicon chromosome
Accession NZ_CP082029
Organism Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K6164_RS19800 Protein ID WP_004142563.1
Coordinates 4067321..4067638 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K6164_RS19795 Protein ID WP_004142561.1
Coordinates 4067041..4067328 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6164_RS19765 (4063022) 4063022..4063270 + 249 WP_020325243.1 DUF1158 domain-containing protein -
K6164_RS19770 (4063288) 4063288..4063629 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K6164_RS19775 (4063660) 4063660..4064775 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
K6164_RS19780 (4064955) 4064955..4065635 + 681 WP_119062547.1 TetR/AcrR family transcriptional regulator -
K6164_RS19785 (4065635) 4065635..4066003 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
K6164_RS19790 (4066123) 4066123..4066776 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
K6164_RS19795 (4067041) 4067041..4067328 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K6164_RS19800 (4067321) 4067321..4067638 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6164_RS19805 (4067823) 4067823..4068866 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
K6164_RS19810 (4069536) 4069536..4070402 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
K6164_RS19815 (4070511) 4070511..4071938 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T214020 WP_004142563.1 NZ_CP082029:c4067638-4067321 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T214020 NZ_CP109939:c2034668-2034411 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT214020 WP_004142561.1 NZ_CP082029:c4067328-4067041 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT214020 NZ_CP109939:c2034930-2034670 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References