Detailed information of TA system TA214002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 82444..83171
• Replicon: plasmid pKP18-2073-1
• Accession: NZ_CP082026
• Organism: Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: K6166_RS29165
• Protein ID: WP_011251285.1
• Coordinates: 82444..82755 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6166_RS29170
• Protein ID: WP_011251286.1
• Coordinates: 82752..83171 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6166_RS29135	77635..78615	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
K6166_RS29140	79161..79340	-	180	Protein_85	IS5/IS1182 family transposase	-
K6166_RS29145	79380..80918	-	1539	WP_017901237.1	IS66 family transposase	-
K6166_RS29150	80967..81314	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
K6166_RS29155	81311..81688	-	378	WP_004118218.1	transposase	-
K6166_RS29160	81802..82239	+	438	Protein_89	DDE-type integrase/transposase/recombinase	-
K6166_RS29165	82444..82755	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
K6166_RS29170	82752..83171	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
K6166_RS29175	83241..83654	+	414	WP_017901337.1	hypothetical protein	-
K6166_RS29180	83700..84668	-	969	WP_074168518.1	IS5-like element IS903B family transposase	-
K6166_RS29185	84772..85038	+	267	WP_017900868.1	hypothetical protein	-
K6166_RS29190	85152..85687	-	536	Protein_95	transposase	-
K6166_RS29195	86212..87579	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-187	-	1..237812	237812
	-	inside	IScluster/Tn	-	-	77635..85370	7735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T214002 WP_011251285.1 NZ_CP082026:82444-82755 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T214002 NZ_CP109933:c1233831-1233514 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT214002 WP_011251286.1 NZ_CP082026:82752-83171 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT214002 NZ_CP109933:c1234043-1233834 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCCTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1