Detailed information of TA system TA213997

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82163..82416
• Replicon: plasmid pKP18-2073-KPC2
• Accession: NZ_CP082025
• Organism: Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K6166_RS28390
• Protein ID: WP_001312851.1
• Coordinates: 82163..82312 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82357..82416 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6166_RS28355 (77522)	77522..77937	-	416	Protein_107	IS1-like element IS1B family transposase	-
K6166_RS28360 (78186)	78186..78587	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K6166_RS28365 (78520)	78520..78777	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K6166_RS28370 (78870)	78870..79523	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K6166_RS28375 (80462)	80462..81319	-	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
K6166_RS30230 (81338)	81338..81516	-	179	Protein_112	protein CopA/IncA	-
K6166_RS28385 (81631)	81631..81879	-	249	WP_000083837.1	replication regulatory protein RepA	-
K6166_RS28390 (82163)	82163..82312	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (82357)	82357..82416	+	60	NuclAT_1	-	Antitoxin
- (82357)	82357..82416	+	60	NuclAT_1	-	Antitoxin
- (82357)	82357..82416	+	60	NuclAT_1	-	Antitoxin
- (82357)	82357..82416	+	60	NuclAT_1	-	Antitoxin
K6166_RS28395 (82617)	82617..82949	-	333	WP_152916585.1	hypothetical protein	-
K6166_RS28400 (83011)	83011..83610	-	600	WP_032083981.1	PIN domain-containing protein	-
K6166_RS28405 (83996)	83996..84196	-	201	WP_015059022.1	hypothetical protein	-
K6166_RS28410 (84328)	84328..84888	-	561	WP_000139328.1	fertility inhibition protein FinO	-
K6166_RS28415 (84943)	84943..85689	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
K6166_RS28420 (85709)	85709..85909	-	201	WP_072354025.1	hypothetical protein	-
K6166_RS28425 (85934)	85934..86638	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6166_RS28430 (86691)	86691..86756	+	66	Protein_122	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-187 / blaKPC-2	-	1..130597	130597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T213997 WP_001312851.1 NZ_CP082025:c82312-82163 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T213997 NZ_CP109933:20010-20186 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTCATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT213997 NZ_CP082025:82357-82416 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin