Detailed information of TA system TA213993

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36185..36454
• Replicon: plasmid pKP18-2073-KPC2
• Accession: NZ_CP082025
• Organism: Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6166_RS28065
• Protein ID: WP_001372321.1
• Coordinates: 36329..36454 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36185..36250 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6166_RS28040	31895..32422	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
K6166_RS28045	32480..32713	+	234	WP_000006003.1	DUF905 family protein	-
K6166_RS28050	32774..34797	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
K6166_RS28055	34866..35300	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K6166_RS28060	35297..36016	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36028..36252	+	225	NuclAT_0	-	-
-	36028..36252	+	225	NuclAT_0	-	-
-	36028..36252	+	225	NuclAT_0	-	-
-	36028..36252	+	225	NuclAT_0	-	-
-	36185..36250	-	66	-	-	Antitoxin
K6166_RS30220	36238..36387	+	150	Protein_48	plasmid maintenance protein Mok	-
K6166_RS28065	36329..36454	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6166_RS28070	36773..37069	-	297	Protein_50	hypothetical protein	-
K6166_RS28075	37369..37665	+	297	WP_001272251.1	hypothetical protein	-
K6166_RS28080	37776..38597	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K6166_RS28085	38894..39541	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K6166_RS28090	39818..40201	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6166_RS28095	40392..41078	+	687	WP_015059009.1	PAS domain-containing protein	-
K6166_RS28100	41172..41399	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-187 / blaKPC-2	-	1..130597	130597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T213993 WP_001372321.1 NZ_CP082025:36329-36454 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T213993 NZ_CP109932:2937463-2938050 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT213993 NZ_CP082025:c36250-36185 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin