Detailed information of TA system TA213969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 86121..86848
• Replicon: plasmid pKP18-2050-1
• Accession: NZ_CP082021
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: K6159_RS29210
• Protein ID: WP_011251285.1
• Coordinates: 86121..86432 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6159_RS29215
• Protein ID: WP_011251286.1
• Coordinates: 86429..86848 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS29180	81312..82292	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
K6159_RS29185	82838..83017	-	180	Protein_90	IS5/IS1182 family transposase	-
K6159_RS29190	83057..84595	-	1539	WP_017901237.1	IS66 family transposase	-
K6159_RS29195	84644..84991	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
K6159_RS29200	84988..85365	-	378	WP_004118218.1	transposase	-
K6159_RS29205	85479..85916	+	438	Protein_94	DDE-type integrase/transposase/recombinase	-
K6159_RS29210	86121..86432	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
K6159_RS29215	86429..86848	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
K6159_RS29220	86918..87331	+	414	WP_017901337.1	hypothetical protein	-
K6159_RS29225	87377..88345	-	969	WP_074168518.1	IS5-like element IS903B family transposase	-
K6159_RS29230	88449..88715	+	267	WP_017900868.1	hypothetical protein	-
K6159_RS29235	88829..89364	-	536	Protein_100	transposase	-
K6159_RS29240	89889..91256	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA5 / qacE / blaSHV-12	-	1..241489	241489
	-	inside	IScluster/Tn	-	-	81312..89047	7735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T213969 WP_011251285.1 NZ_CP082021:86121-86432 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T213969 NZ_CP109929:2228028-2228130 [Escherichia coli O6:K2:H1]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT213969 WP_011251286.1 NZ_CP082021:86429-86848 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT213969 NZ_CP109929:c2228168-2227900 [Escherichia coli O6:K2:H1]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1