Detailed information of TA system TA213962

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37150..37419
• Replicon: plasmid pKP18-2050-KPC2
• Accession: NZ_CP082020
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6159_RS28075
• Protein ID: WP_001372321.1
• Coordinates: 37294..37419 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37150..37215 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS28050	32860..33387	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
K6159_RS28055	33445..33678	+	234	WP_000006003.1	DUF905 family protein	-
K6159_RS28060	33739..35762	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
K6159_RS28065	35831..36265	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K6159_RS28070	36262..36981	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36993..37217	+	225	NuclAT_0	-	-
-	36993..37217	+	225	NuclAT_0	-	-
-	36993..37217	+	225	NuclAT_0	-	-
-	36993..37217	+	225	NuclAT_0	-	-
-	37150..37215	-	66	-	-	Antitoxin
K6159_RS30270	37203..37352	+	150	Protein_48	plasmid maintenance protein Mok	-
K6159_RS28075	37294..37419	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6159_RS28080	37738..38034	-	297	Protein_50	hypothetical protein	-
K6159_RS28085	38334..38630	+	297	WP_001272251.1	hypothetical protein	-
K6159_RS28090	38741..39562	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K6159_RS28095	39859..40506	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K6159_RS28100	40783..41166	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6159_RS28105	41357..42043	+	687	WP_015059009.1	PAS domain-containing protein	-
K6159_RS28110	42137..42364	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..135837	135837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T213962 WP_001372321.1 NZ_CP082020:37294-37419 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T213962 NZ_CP109929:c623187-622723 [Escherichia coli O6:K2:H1]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT213962 NZ_CP082020:c37215-37150 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin