Detailed information of TA system TA213936

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57859..58128
• Replicon: plasmid pKP18-238-KPC2
• Accession: NZ_CP082015
• Organism: Klebsiella pneumoniae strain KP18-238

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6162_RS28080
• Protein ID: WP_001372321.1
• Coordinates: 58003..58128 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	57859..57924 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6162_RS28055	53569..54096	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
K6162_RS28060	54154..54387	+	234	WP_000006003.1	DUF905 family protein	-
K6162_RS28065	54448..56471	+	2024	Protein_76	ParB/RepB/Spo0J family partition protein	-
K6162_RS28070	56540..56974	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K6162_RS28075	56971..57690	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	57702..57926	+	225	NuclAT_0	-	-
-	57702..57926	+	225	NuclAT_0	-	-
-	57702..57926	+	225	NuclAT_0	-	-
-	57702..57926	+	225	NuclAT_0	-	-
-	57859..57924	-	66	-	-	Antitoxin
K6162_RS29430	57912..58061	+	150	Protein_79	plasmid maintenance protein Mok	-
K6162_RS28080	58003..58128	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6162_RS28085	58447..58743	-	297	Protein_81	hypothetical protein	-
K6162_RS28090	59043..59339	+	297	WP_001272251.1	hypothetical protein	-
K6162_RS28095	59450..60271	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K6162_RS28100	60568..61215	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K6162_RS28105	61492..61875	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6162_RS28110	62066..62752	+	687	WP_015059009.1	PAS domain-containing protein	-
K6162_RS28115	62846..63073	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..128480	128480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T213936 WP_001372321.1 NZ_CP082015:58003-58128 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T213936 NZ_CP109921:c4394875-4394702 [Escherichia coli O2:K2:H1]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT213936 NZ_CP082015:c57924-57859 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin