Detailed information of TA system TA213933

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20593..20846
• Replicon: plasmid pKP18-238-KPC2
• Accession: NZ_CP082015
• Organism: Klebsiella pneumoniae strain KP18-238

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K6162_RS27840
• Protein ID: WP_001312851.1
• Coordinates: 20697..20846 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	20593..20652 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6162_RS27800 (16253)	16253..16318	-	66	Protein_22	helix-turn-helix domain-containing protein	-
K6162_RS27805 (16371)	16371..17075	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6162_RS27810 (17100)	17100..17300	+	201	WP_072354025.1	hypothetical protein	-
K6162_RS27815 (17320)	17320..18066	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
K6162_RS27820 (18121)	18121..18681	+	561	WP_000139328.1	fertility inhibition protein FinO	-
K6162_RS27825 (18813)	18813..19013	+	201	WP_015059022.1	hypothetical protein	-
K6162_RS27830 (19399)	19399..19998	+	600	WP_032083981.1	PIN domain-containing protein	-
K6162_RS27835 (20060)	20060..20392	+	333	WP_152916585.1	hypothetical protein	-
- (20593)	20593..20652	-	60	NuclAT_1	-	Antitoxin
- (20593)	20593..20652	-	60	NuclAT_1	-	Antitoxin
- (20593)	20593..20652	-	60	NuclAT_1	-	Antitoxin
- (20593)	20593..20652	-	60	NuclAT_1	-	Antitoxin
K6162_RS27840 (20697)	20697..20846	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K6162_RS27845 (21130)	21130..21378	+	249	WP_000083837.1	replication regulatory protein RepA	-
K6162_RS29410 (21493)	21493..21671	+	179	Protein_32	protein CopA/IncA	-
K6162_RS27855 (21690)	21690..22547	+	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
K6162_RS27860 (23486)	23486..24139	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K6162_RS27865 (24232)	24232..24489	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K6162_RS27870 (24422)	24422..24823	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K6162_RS27875 (25072)	25072..25487	+	416	Protein_37	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..128480	128480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T213933 WP_001312851.1 NZ_CP082015:20697-20846 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T213933 NZ_CP109921:4073547-4073699 [Escherichia coli O2:K2:H1]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT213933 NZ_CP082015:c20652-20593 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin