Detailed information of TA system TA021391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3732996..3733653
• Replicon: chromosome
• Accession: NC_007651
• Organism: Burkholderia thailandensis E264

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6L6G8
• Locus tag: BTH_RS28865
• Protein ID: WP_009910242.1
• Coordinates: 3732996..3733328 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BTH_RS32665
• Protein ID: WP_011402654.1
• Coordinates: 3733336..3733653 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTH_RS28840	3729385..3729966	+	582	WP_043300356.1	hypothetical protein	-
BTH_RS28845	3730425..3731204	+	780	WP_011402651.1	hypothetical protein	-
BTH_RS28860	3732412..3732954	+	543	WP_102860125.1	site-specific integrase	-
BTH_RS28865	3732996..3733328	+	333	WP_009910242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTH_RS32665	3733336..3733653	+	318	WP_011402654.1	helix-turn-helix domain-containing protein	Antitoxin
BTH_RS32670	3733905..3734393	+	489	Protein_3285	site-specific integrase	-
BTH_RS28875	3734507..3735472	-	966	Protein_3286	IS256 family transposase	-
BTH_RS28880	3735552..3736112	+	561	Protein_3287	LysR family transcriptional regulator	-
BTH_RS32675	3736078..3736626	+	549	WP_080511571.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
BTH_RS28885	3736559..3737350	-	792	WP_009910252.1	ethanolamine ammonia-lyase subunit EutC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3731605..3735496	3891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-97)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12849.92 Da
• Isoelectric Point: 10.3619

>T21391 WP_009910242.1 NC_007651:3732996-3733328 [Burkholderia thailandensis E264]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T21391 NC_007651:3732996-3733328 [Burkholderia thailandensis E264]
ATGAAATTTGATTGGACGGTCGTCTACTACAACGAACGGGTGAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTCGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAATTCGGGGCGGATCTACGGATGCCGCATTCCAGAGCAATGGGTAACG
GGCTGTTTGAGTTGCGCCCCAAGGGTAGGGAAGGCATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTT
GTGCTGCACTCCTTCGTCAAGAAGACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11277.80 Da
• Isoelectric Point: 10.2764

>AT21391 WP_011402654.1 NC_007651:3733336-3733653 [Burkholderia thailandensis E264]
VKRVRAEGFNPVPHTADDTARLLAGRNVKAAYDALEDEYTALRAILSARRDAGLTQAQIAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 318 bp

>AT21391 NC_007651:3733336-3733653 [Burkholderia thailandensis E264]
GTGAAGCGTGTTCGCGCTGAGGGATTCAATCCGGTTCCGCATACGGCGGACGACACGGCGCGCCTGCTCGCCGGTCGCAA
TGTCAAGGCGGCGTATGACGCGTTGGAGGATGAGTACACCGCGTTGCGCGCTATCTTGTCTGCACGGCGCGATGCTGGTC
TGACACAGGCTCAGATTGCTGAGCGGATGGGTACGACCGCATCGGCGGTCTCGCGGCTTGAGGCTTCGTTGTCGAGTGAA
AAGCATTCACCATCATTTGCGACGCTGCGTAAATACGCGGCTGCATGTGGTAAGCGGCTTGTGATTTCGTTTGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L6G8