Detailed information of TA system TA213909

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89763..90189
• Replicon: plasmid pKP18-41-KPC2
• Accession: NZ_CP082012
• Organism: Klebsiella pneumoniae strain KP18-41

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6147_RS28450
• Protein ID: WP_001372321.1
• Coordinates: 89763..89888 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89965..90189 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6147_RS28415 (84818)	84818..85045	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
K6147_RS28420 (85139)	85139..85825	-	687	WP_015059009.1	PAS domain-containing protein	-
K6147_RS28425 (86016)	86016..86399	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6147_RS28430 (86676)	86676..87323	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K6147_RS28435 (87620)	87620..88441	-	822	WP_001234445.1	DUF932 domain-containing protein	-
K6147_RS28440 (88552)	88552..88848	-	297	WP_001272251.1	hypothetical protein	-
K6147_RS28445 (89148)	89148..89444	+	297	Protein_115	hypothetical protein	-
K6147_RS28450 (89763)	89763..89888	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6147_RS29100 (89830)	89830..89979	-	150	Protein_117	plasmid maintenance protein Mok	-
- (89965)	89965..90189	-	225	NuclAT_0	-	Antitoxin
- (89965)	89965..90189	-	225	NuclAT_0	-	Antitoxin
- (89965)	89965..90189	-	225	NuclAT_0	-	Antitoxin
- (89965)	89965..90189	-	225	NuclAT_0	-	Antitoxin
K6147_RS28455 (90201)	90201..90920	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
K6147_RS28460 (90917)	90917..91351	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K6147_RS28465 (91420)	91420..93443	-	2024	Protein_120	ParB/RepB/Spo0J family partition protein	-
K6147_RS28470 (93504)	93504..93737	-	234	WP_000006003.1	DUF905 family protein	-
K6147_RS28475 (93795)	93795..94322	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
K6147_RS29105 (94624)	94624..95091	+	468	Protein_123	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B	-	1..153214	153214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T213909 WP_001372321.1 NZ_CP082012:c89888-89763 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T213909 NZ_CP109919:c4640942-4640577 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT213909 NZ_CP082012:c90189-89965 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin