Detailed information of TA system TA213856

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68345..68598
• Replicon: plasmid pKP18-1-KPC2
• Accession: NZ_CP082003
• Organism: Klebsiella pneumoniae strain KP18-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K6149_RS28310
• Protein ID: WP_001312851.1
• Coordinates: 68449..68598 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68345..68404 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6149_RS28270 (64005)	64005..64070	-	66	Protein_76	helix-turn-helix domain-containing protein	-
K6149_RS28275 (64123)	64123..64827	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6149_RS28280 (64852)	64852..65052	+	201	WP_072354025.1	hypothetical protein	-
K6149_RS28285 (65072)	65072..65818	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
K6149_RS28290 (65873)	65873..66433	+	561	WP_000139328.1	fertility inhibition protein FinO	-
K6149_RS28295 (66565)	66565..66765	+	201	WP_015059022.1	hypothetical protein	-
K6149_RS28300 (67151)	67151..67750	+	600	WP_032083981.1	PIN domain-containing protein	-
K6149_RS28305 (67812)	67812..68144	+	333	WP_152916585.1	hypothetical protein	-
- (68345)	68345..68404	-	60	NuclAT_1	-	Antitoxin
- (68345)	68345..68404	-	60	NuclAT_1	-	Antitoxin
- (68345)	68345..68404	-	60	NuclAT_1	-	Antitoxin
- (68345)	68345..68404	-	60	NuclAT_1	-	Antitoxin
K6149_RS28310 (68449)	68449..68598	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K6149_RS28315 (68882)	68882..69130	+	249	WP_000083837.1	replication regulatory protein RepA	-
K6149_RS29115 (69245)	69245..69423	+	179	Protein_86	protein CopA/IncA	-
K6149_RS28325 (69442)	69442..70299	+	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
K6149_RS28330 (71238)	71238..71891	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K6149_RS28335 (71984)	71984..72241	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K6149_RS28340 (72174)	72174..72575	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K6149_RS28345 (72824)	72824..73239	+	416	Protein_91	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B	-	1..153399	153399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T213856 WP_001312851.1 NZ_CP082003:68449-68598 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T213856 NZ_CP109890:1157858-1158163 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT213856 NZ_CP082003:c68404-68345 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin