Detailed information of TA system TA213820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1017919..1018498
• Replicon: chromosome
• Accession: NZ_CP081946
• Organism: Pseudomonas sp. DR48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K5K95_RS04640
• Protein ID: WP_222588549.1
• Coordinates: 1017919..1018197 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5K95_RS04645
• Protein ID: WP_222588550.1
• Coordinates: 1018211..1018498 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5K95_RS04625 (K5K95_04625)	1014003..1016204	-	2202	WP_222588546.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K5K95_RS04630 (K5K95_04630)	1016201..1017190	-	990	WP_222588547.1	xanthine dehydrogenase family protein subunit M	-
K5K95_RS04635 (K5K95_04635)	1017187..1017717	-	531	WP_222588548.1	2Fe-2S iron-sulfur cluster-binding protein	-
K5K95_RS04640 (K5K95_04640)	1017919..1018197	+	279	WP_222588549.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5K95_RS04645 (K5K95_04645)	1018211..1018498	+	288	WP_222588550.1	HigA family addiction module antitoxin	Antitoxin
K5K95_RS04650 (K5K95_04650)	1018505..1019056	-	552	WP_222588551.1	DJ-1/PfpI family protein	-
K5K95_RS04655 (K5K95_04655)	1019124..1019507	-	384	WP_261398170.1	YolA family protein	-
K5K95_RS04660 (K5K95_04660)	1019788..1021083	+	1296	WP_222588552.1	NCS2 family permease	-
K5K95_RS04665 (K5K95_04665)	1021080..1022159	+	1080	WP_222588553.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
K5K95_RS04670 (K5K95_04670)	1022210..1022656	-	447	WP_222588554.1	DUF2442 domain-containing protein	-
K5K95_RS04675 (K5K95_04675)	1022653..1023081	-	429	WP_222588555.1	DUF4160 domain-containing protein	-
K5K95_RS04680 (K5K95_04680)	1023292..1023498	-	207	WP_208671354.1	4-oxalocrotonate tautomerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-91)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10753.33 Da
• Isoelectric Point: 9.5306

>T213820 WP_222588549.1 NZ_CP081946:1017919-1018197 [Pseudomonas sp. DR48]
MIRSFQHKGLRGFYETGSTRGIRADHAKRLSRMLQFMDRAVLPGDLDLPGWRLHPLKGELADYWSLSVSGNWRVIFRFIG
SDIELVDYLDYH

Nucleotide

• Length: 279 bp

>T213820 NZ_CP109874:3041511-3042005 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10465.01 Da
• Isoelectric Point: 6.6962

>AT213820 WP_222588550.1 NZ_CP081946:1018211-1018498 [Pseudomonas sp. DR48]
MPLHNPPHPGETLLTDVLPELGISVAELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGVQTDYDLWQA
EHREQPAIEPIAAHA

Nucleotide

• Length: 288 bp

>AT213820 NZ_CP109874:3041221-3041514 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA