Detailed information of TA system TA213812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3990920..3991583
• Replicon: chromosome
• Accession: NZ_CP081943
• Organism: Pseudomonas sp. MM213

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K5R88_RS18215
• Protein ID: WP_223452637.1
• Coordinates: 3991218..3991583 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5R88_RS18210
• Protein ID: WP_008027032.1
• Coordinates: 3990920..3991225 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5R88_RS18200 (K5R88_18095)	3987002..3990748	+	3747	WP_223452638.1	bifunctional diguanylate cyclase/phosphodiesterase	-
K5R88_RS18210 (K5R88_18105)	3990920..3991225	-	306	WP_008027032.1	XRE family transcriptional regulator	Antitoxin
K5R88_RS18215 (K5R88_18110)	3991218..3991583	-	366	WP_223452637.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5R88_RS18220 (K5R88_18115)	3992077..3992385	+	309	WP_223452636.1	DUF3077 domain-containing protein	-
K5R88_RS18225 (K5R88_18120)	3992455..3993102	-	648	WP_223452654.1	peptidase M41	-
K5R88_RS18230 (K5R88_18125)	3993474..3994472	+	999	WP_223452635.1	Abi family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13822.47 Da
• Isoelectric Point: 5.2361

>T213812 WP_223452637.1 NZ_CP081943:c3991583-3991218 [Pseudomonas sp. MM213]
MGWEIEYTDEFEGWWESLDAKEQSSVSASVDLLGLLGPGLRFPHSSDIKGSRLGNLRELRVQHAGRPIRVLYAFDPRRCA
LLLIGGDKTGQNRWYDEHVPLAEKLYDDHLETLRKEGRNDG

Nucleotide

• Length: 366 bp

>T213812 NZ_CP109874:934517-934924 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11747.59 Da
• Isoelectric Point: 9.6910

>AT213812 WP_008027032.1 NZ_CP081943:c3991225-3990920 [Pseudomonas sp. MM213]
MAKKFAELQARMTPESRANAERLFQQHLKEMPLHELRKAQELSQETLAKMLHVNQAAISRMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVKIENFAN

Nucleotide

• Length: 306 bp

>AT213812 NZ_CP109874:934270-934536 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA