Detailed information of TA system TA213772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3797228..3797825
• Replicon: chromosome
• Accession: NZ_CP081896
• Organism: Klebsiella pneumoniae strain PA-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KZ665_RS18745
• Protein ID: WP_004893639.1
• Coordinates: 3797508..3797825 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KZ665_RS18740
• Protein ID: WP_004142561.1
• Coordinates: 3797228..3797515 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ665_RS18710 (KZ665_18655)	3793308..3793556	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KZ665_RS18715 (KZ665_18660)	3793574..3793915	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KZ665_RS18720 (KZ665_18665)	3793946..3795061	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
KZ665_RS18725 (KZ665_18670)	3795241..3795822	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
KZ665_RS18730 (KZ665_18675)	3795822..3796190	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KZ665_RS18735 (KZ665_18680)	3796310..3796963	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KZ665_RS18740 (KZ665_18685)	3797228..3797515	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KZ665_RS18745 (KZ665_18690)	3797508..3797825	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ665_RS18750 (KZ665_18695)	3798010..3799053	-	1044	WP_223051097.1	DUF2157 domain-containing protein	-
KZ665_RS18755 (KZ665_18700)	3799723..3800589	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KZ665_RS18760 (KZ665_18705)	3800698..3802125	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T213772 WP_004893639.1 NZ_CP081896:c3797825-3797508 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T213772 NZ_CP109850:c755646-754777 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACATCCCCGCCCCGAGCCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTGCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCACGCCTGGAGCTGCTGCTGGATGCTCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGTCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCCTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGCCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT213772 WP_004142561.1 NZ_CP081896:c3797515-3797228 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT213772 NZ_CP109850:c754784-753951 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACTCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTACGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTCTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAACTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTACGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1