Detailed information of TA system TA021371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 985389..985993
• Replicon: chromosome
• Accession: NC_007626
• Organism: Magnetospirillum magneticum AMB-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q2W8V5
• Locus tag: AMB_RS04710
• Protein ID: WP_011383357.1
• Coordinates: 985389..985715 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AMB_RS23915
• Protein ID: WP_008620724.1
• Coordinates: 985712..985993 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMB_RS23155	981730..982824	+	1095	WP_050750625.1	two-component sensor histidine kinase	-
AMB_RS04700	983206..983484	-	279	WP_011383355.1	transposase	-
AMB_RS23905	983699..984457	-	759	WP_083763424.1	EAL domain-containing protein	-
AMB_RS23910	984585..985049	-	465	WP_083763425.1	DUF1465 family protein	-
AMB_RS04710	985389..985715	+	327	WP_011383357.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMB_RS23915	985712..985993	+	282	WP_008620724.1	helix-turn-helix domain-containing protein	Antitoxin
AMB_RS23920	986144..986539	-	396	WP_083763426.1	transcriptional repressor	-
AMB_RS04720	986845..987225	+	381	WP_008622021.1	response regulator	-
AMB_RS04725	987311..987772	-	462	WP_011383360.1	transcriptional regulator NrdR	-
AMB_RS04735	988098..989627	+	1530	WP_083763427.1	diguanylate cyclase	-
AMB_RS04740	989744..990139	+	396	WP_043743454.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	985389..996529	11140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-82)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12249.59 Da
• Isoelectric Point: 10.5339

>T21371 WP_011383357.1 NC_007626:985389-985715 [Magnetospirillum magneticum AMB-1]
MEWKVETLNEIVDAEIAVLPKDMQARFLRIAGMIETAGLEGMGHPHVKPLEGKLWEMRLMGRDGIARALYVTVTGRRVVV
VRAFVKKTQKTPRQEIELALARAKEILK

Nucleotide

• Length: 327 bp

>T21371 NC_007626:985389-985715 [Magnetospirillum magneticum AMB-1]
ATGGAATGGAAGGTCGAAACCCTCAACGAAATCGTGGACGCGGAAATCGCTGTCTTGCCCAAGGATATGCAGGCCCGTTT
TCTGCGGATAGCCGGGATGATTGAGACCGCTGGGTTGGAAGGTATGGGGCATCCTCATGTGAAGCCGCTTGAGGGCAAGT
TATGGGAGATGCGGCTAATGGGCCGCGACGGCATCGCCCGTGCCCTCTATGTGACCGTCACTGGTCGGCGGGTCGTGGTA
GTGCGGGCCTTTGTAAAAAAGACTCAGAAGACGCCTCGCCAGGAGATTGAACTCGCATTGGCTCGGGCCAAGGAGATTTT
GAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10131.62 Da
• Isoelectric Point: 10.5386

>AT21371 WP_008620724.1 NC_007626:985712-985993 [Magnetospirillum magneticum AMB-1]
MTSLNDLKAKMLADPEVKAEYDRLAPEFEIAETLIRARQRAGLSQAEVAKRMGTTQSVVARLESGRSLPSSTSLARYAAA
TGSRLRVELLPGR

Nucleotide

• Length: 282 bp

>AT21371 NC_007626:985712-985993 [Magnetospirillum magneticum AMB-1]
ATGACCAGCCTGAATGATCTCAAGGCTAAAATGCTGGCTGATCCCGAGGTTAAGGCCGAATATGACCGTCTCGCTCCCGA
GTTCGAGATTGCGGAAACCCTTATCCGTGCCCGGCAACGGGCGGGTCTATCTCAGGCCGAAGTCGCAAAAAGGATGGGCA
CCACCCAGTCCGTGGTTGCACGGCTGGAAAGTGGTCGTTCCCTTCCTTCATCGACTTCACTGGCACGCTACGCCGCCGCC
ACAGGAAGCCGCCTTCGTGTTGAGCTTCTTCCTGGTCGATGA

Structures

Toxin

• AlphaFold DB: Q2W8V5