Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3882772..3883369 Replicon chromosome
Accession NZ_CP081892
Organism Klebsiella pneumoniae strain NY-06

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KZ657_RS19225 Protein ID WP_004142563.1
Coordinates 3883052..3883369 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KZ657_RS19220 Protein ID WP_004142561.1
Coordinates 3882772..3883059 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ657_RS19190 (3878979) 3878979..3879227 + 249 WP_016529803.1 DUF1158 domain-containing protein -
KZ657_RS19195 (3879245) 3879245..3879586 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KZ657_RS19200 (3879617) 3879617..3880732 - 1116 WP_167875462.1 MBL fold metallo-hydrolase -
KZ657_RS19205 (3880912) 3880912..3881496 + 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
KZ657_RS19210 (3881493) 3881493..3881861 + 369 WP_002893024.1 MmcQ/YjbR family DNA-binding protein -
KZ657_RS19215 (3881981) 3881981..3882634 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KZ657_RS19220 (3882772) 3882772..3883059 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KZ657_RS19225 (3883052) 3883052..3883369 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZ657_RS19230 (3883554) 3883554..3884597 - 1044 WP_048336957.1 DUF2157 domain-containing protein -
KZ657_RS19235 (3885263) 3885263..3886129 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KZ657_RS19240 (3886238) 3886238..3887665 + 1428 WP_023300814.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T213709 WP_004142563.1 NZ_CP081892:c3883369-3883052 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T213709 NZ_CP109824:c4434773-4434498 [Serratia marcescens]
ATGTTAACCGTTAAATGGACTGACGAAGCAAAAACGGGTTTATATACGCTGCTGGCTTTTATCGCTGAGCGAAATCCACT
TGCCGCTGAGGCTTTGTTGCAACGTATCGAAGAGTCGGTTATCCCGTTAGCGGAACATCCTTATCTGTTCCGGCCTGGCA
GGGTGAACGGGACTCGTGAAATGGTTGTTCATCCTAATTACATTGTGGTTTACCGCGTGCTGGCTGCACATATTGAAATA
GTGAGTGTCCTGCACGCTCGTCAGGAATACCCTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT213709 WP_004142561.1 NZ_CP081892:c3883059-3882772 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT213709 NZ_CP109824:c4434970-4434779 [Serratia marcescens]
ATGCCGACAAAATTGGATCCCATCGTCTCTGAGTTTGAGACTCAAGAACAGGCTGATAGCTACGATCGTTGGTTCAGAAG
CACCGTGGAATCTTCCGTCAATAGCGACACCCCCCGTATCCCCCATGATGAAGTCATGGCTGGTGCTCGTCACATTATCG
AGCAGGCGAAAGCCAAAAGAAAATCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References