Detailed information of TA system TA021370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 712501..713076
• Replicon: chromosome
• Accession: NC_007626
• Organism: Magnetospirillum magneticum AMB-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q2W9L0
• Locus tag: AMB_RS03400
• Protein ID: WP_011383104.1
• Coordinates: 712798..713076 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AMB_RS03395
• Protein ID: WP_011383103.1
• Coordinates: 712501..712788 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMB_RS03385	708705..710666	+	1962	WP_158303921.1	hypothetical protein	-
AMB_RS24835	711516..712160	-	645	WP_148207282.1	hypothetical protein	-
AMB_RS03390	712256..712492	+	237	WP_011383102.1	helix-turn-helix domain-containing protein	-
AMB_RS03395	712501..712788	-	288	WP_011383103.1	HigA family addiction module antidote protein	Antitoxin
AMB_RS03400	712798..713076	-	279	WP_011383104.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMB_RS03405	713495..714388	-	894	WP_011383105.1	LysR family transcriptional regulator	-
AMB_RS03410	714464..715414	-	951	WP_011383106.1	thioredoxin-disulfide reductase	-
AMB_RS03415	715512..716135	-	624	WP_011383107.1	peroxiredoxin	-
AMB_RS03420	716405..716905	+	501	WP_011383108.1	Lrp/AsnC family transcriptional regulator	-
AMB_RS03425	716928..717689	+	762	WP_011383109.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	694072..713076	19004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10493.85 Da
• Isoelectric Point: 7.3590

>T21370 WP_011383104.1 NC_007626:c713076-712798 [Magnetospirillum magneticum AMB-1]
MIASFHDKETERLYQAGTSKRFAAIAKAALRKLDMLDAAAELQDLSSPPGNRLEALEGDRKGQHSIRINDQWRLCFIWRD
GAAHRVEITDYH

Nucleotide

• Length: 279 bp

>T21370 NC_007626:c713076-712798 [Magnetospirillum magneticum AMB-1]
ATGATAGCCAGCTTTCACGACAAGGAAACGGAGCGCCTATATCAGGCGGGCACGTCCAAGCGGTTTGCCGCTATCGCCAA
AGCCGCGTTACGCAAGCTGGACATGCTTGATGCGGCTGCCGAGTTGCAAGACCTCTCCTCACCCCCAGGGAACCGCCTTG
AGGCTCTGGAGGGCGACAGGAAGGGCCAACACAGCATCCGTATCAATGACCAGTGGCGGCTGTGTTTTATATGGCGTGAT
GGTGCCGCGCACCGTGTCGAGATCACCGACTACCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10446.00 Da
• Isoelectric Point: 8.7393

>AT21370 WP_011383103.1 NC_007626:c712788-712501 [Magnetospirillum magneticum AMB-1]
MRIKTHPGEILREEFMAPLGLSANALATALRVPATRIGEIIKEHRSITADTALRLARYFGTTPQFWLNLQSAHDLSKVEA
EAGDKIAHDVTPRAA

Nucleotide

• Length: 288 bp

>AT21370 NC_007626:c712788-712501 [Magnetospirillum magneticum AMB-1]
ATGCGCATCAAGACCCATCCCGGTGAAATCCTCCGCGAAGAATTCATGGCCCCCTTGGGGCTGTCGGCCAACGCCCTTGC
CACGGCGTTACGCGTCCCGGCAACCCGCATTGGCGAGATCATCAAGGAGCACCGCTCCATCACCGCCGATACGGCTTTGC
GGTTGGCCCGGTATTTCGGCACCACGCCACAATTCTGGCTCAACCTCCAAAGCGCCCATGACCTGTCAAAGGTGGAAGCT
GAGGCCGGAGACAAGATTGCACACGACGTGACGCCACGGGCGGCGTAG

Structures

Toxin

• AlphaFold DB: Q2W9L0