Detailed information of TA system TA213689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3882446..3883043
• Replicon: chromosome
• Accession: NZ_CP081891
• Organism: Klebsiella pneumoniae strain CT-04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KZ663_RS18960
• Protein ID: WP_004893639.1
• Coordinates: 3882726..3883043 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KZ663_RS18955
• Protein ID: WP_004142561.1
• Coordinates: 3882446..3882733 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ663_RS18925 (KZ663_18865)	3878526..3878774	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KZ663_RS18930 (KZ663_18870)	3878792..3879133	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KZ663_RS18935 (KZ663_18875)	3879164..3880279	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
KZ663_RS18940 (KZ663_18880)	3880459..3881040	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
KZ663_RS18945 (KZ663_18885)	3881040..3881408	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KZ663_RS18950 (KZ663_18890)	3881528..3882181	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KZ663_RS18955 (KZ663_18895)	3882446..3882733	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KZ663_RS18960 (KZ663_18900)	3882726..3883043	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ663_RS18965 (KZ663_18905)	3883228..3884271	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
KZ663_RS18970 (KZ663_18910)	3884937..3885803	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KZ663_RS18975 (KZ663_18915)	3885912..3887339	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T213689 WP_004893639.1 NZ_CP081891:c3883043-3882726 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T213689 NZ_CP109795:c1346864-1346118 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCACTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT213689 WP_004142561.1 NZ_CP081891:c3882733-3882446 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT213689 NZ_CP109795:c1346117-1345947 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1