Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331650..332236 Replicon chromosome
Accession NZ_CP081891
Organism Klebsiella pneumoniae strain CT-04

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KZ663_RS01545 Protein ID WP_002920800.1
Coordinates 331868..332236 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KZ663_RS01540 Protein ID WP_004174006.1
Coordinates 331650..331871 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ663_RS01520 (KZ663_01515) 327807..328733 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KZ663_RS01525 (KZ663_01520) 328730..330007 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KZ663_RS01530 (KZ663_01525) 330004..330771 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KZ663_RS01535 (KZ663_01530) 330773..331486 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KZ663_RS01540 (KZ663_01535) 331650..331871 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KZ663_RS01545 (KZ663_01540) 331868..332236 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KZ663_RS01550 (KZ663_01545) 332509..333825 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KZ663_RS01555 (KZ663_01550) 333932..334819 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KZ663_RS01560 (KZ663_01555) 334816..335661 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KZ663_RS01565 (KZ663_01560) 335663..336733 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 328730..337470 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T213676 WP_002920800.1 NZ_CP081891:331868-332236 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T213676 NZ_CP109775:c1752116-1752009 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT213676 WP_004174006.1 NZ_CP081891:331650-331871 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT213676 NZ_CP109775:1752164-1752230 [Shigella sonnei]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References