Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3790816..3791413 Replicon chromosome
Accession NZ_CP081889
Organism Klebsiella pneumoniae strain IA-044

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KZ664_RS18610 Protein ID WP_004142563.1
Coordinates 3791096..3791413 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KZ664_RS18605 Protein ID WP_004142561.1
Coordinates 3790816..3791103 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ664_RS18575 (KZ664_18540) 3786896..3787144 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KZ664_RS18580 (KZ664_18545) 3787162..3787503 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KZ664_RS18585 (KZ664_18550) 3787534..3788649 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KZ664_RS18590 (KZ664_18555) 3788829..3789410 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
KZ664_RS18595 (KZ664_18560) 3789410..3789778 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KZ664_RS18600 (KZ664_18565) 3789898..3790551 + 654 WP_094833297.1 oxygen-insensitive NAD(P)H nitroreductase -
KZ664_RS18605 (KZ664_18570) 3790816..3791103 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KZ664_RS18610 (KZ664_18575) 3791096..3791413 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZ664_RS18615 (KZ664_18580) 3791598..3792641 - 1044 WP_094833296.1 DUF2157 domain-containing protein -
KZ664_RS18620 (KZ664_18585) 3793307..3794173 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KZ664_RS18625 (KZ664_18590) 3794282..3795709 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T213652 WP_004142563.1 NZ_CP081889:c3791413-3791096 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T213652 NZ_CP109755:135902-136387 [Enterobacter roggenkampii]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT213652 WP_004142561.1 NZ_CP081889:c3791103-3790816 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT213652 NZ_CP109755:135648-135914 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References