Detailed information of TA system TA213636

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68912..69176
• Replicon: plasmid unnamed3
• Accession: NZ_CP081888
• Organism: Escherichia coli strain WW2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: K5O11_RS24895
• Protein ID: WP_024179505.1
• Coordinates: 68912..69064 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	69116..69176 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O11_RS24870 (64100)	64100..66268	+	2169	WP_130723489.1	DotA/TraY family protein	-
K5O11_RS24875 (66333)	66333..66995	+	663	WP_124046891.1	plasmid IncI1-type surface exclusion protein ExcA	-
K5O11_RS24880 (67090)	67090..67299	+	210	WP_001140544.1	hemolysin expression modulator Hha	-
K5O11_RS24885 (67482)	67482..67658	+	177	WP_001054900.1	hypothetical protein	-
- (68144)	68144..68195	+	52	NuclAT_1	-	-
- (68144)	68144..68195	+	52	NuclAT_1	-	-
- (68144)	68144..68195	+	52	NuclAT_1	-	-
- (68144)	68144..68195	+	52	NuclAT_1	-	-
K5O11_RS24890 (68589)	68589..68840	+	252	WP_024171163.1	hypothetical protein	-
K5O11_RS24895 (68912)	68912..69064	-	153	WP_024179505.1	Hok/Gef family protein	Toxin
- (69116)	69116..69176	+	61	NuclAT_0	-	Antitoxin
- (69116)	69116..69176	+	61	NuclAT_0	-	Antitoxin
- (69116)	69116..69176	+	61	NuclAT_0	-	Antitoxin
- (69116)	69116..69176	+	61	NuclAT_0	-	Antitoxin
K5O11_RS24900 (69356)	69356..70564	+	1209	WP_024171190.1	IncI1-type conjugal transfer protein TrbA	-
K5O11_RS24905 (70583)	70583..71653	+	1071	WP_124046954.1	IncI1-type conjugal transfer protein TrbB	-
K5O11_RS24910 (71646)	71646..73937	+	2292	WP_222563290.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103282	103282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5743.07 Da
• Isoelectric Point: 8.7948

>T213636 WP_024179505.1 NZ_CP081888:c69064-68912 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWIVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T213636 NZ_CP109744:3380859-3380962 [Citrobacter portucalensis]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 61 bp

>AT213636 NZ_CP081888:69116-69176 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin