Detailed information of TA system TA213631

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72272..72526
• Replicon: plasmid unnamed2
• Accession: NZ_CP081887
• Organism: Escherichia coli strain WW2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K5O11_RS24320
• Protein ID: WP_001312851.1
• Coordinates: 72272..72421 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	72465..72526 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O11_RS24290 (67519)	67519..68430	-	912	WP_000440183.1	carbamate kinase	-
K5O11_RS24295 (68441)	68441..69661	-	1221	WP_000410951.1	arginine deiminase	-
K5O11_RS24300 (70368)	70368..70982	+	615	Protein_81	VENN motif pre-toxin domain-containing protein	-
K5O11_RS24305 (70982)	70982..71428	-	447	Protein_82	plasmid replication initiator RepA	-
K5O11_RS24310 (71421)	71421..71495	-	75	WP_032336874.1	RepA leader peptide Tap	-
K5O11_RS24315 (71731)	71731..71988	-	258	WP_000083833.1	replication regulatory protein RepA	-
K5O11_RS24320 (72272)	72272..72421	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (72465)	72465..72526	+	62	NuclAT_0	-	Antitoxin
- (72465)	72465..72526	+	62	NuclAT_0	-	Antitoxin
- (72465)	72465..72526	+	62	NuclAT_0	-	Antitoxin
- (72465)	72465..72526	+	62	NuclAT_0	-	Antitoxin
K5O11_RS24325 (72665)	72665..72847	-	183	WP_000968309.1	hypothetical protein	-
K5O11_RS24330 (72948)	72948..73564	+	617	Protein_87	IS1-like element IS1A family transposase	-
K5O11_RS24335 (73602)	73602..75173	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
K5O11_RS24340 (75193)	75193..75540	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
K5O11_RS24345 (75540)	75540..76217	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
K5O11_RS24350 (76272)	76272..76361	+	90	Protein_91	IS1 family transposase	-
K5O11_RS24355 (76662)	76662..76874	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sitABCD / blaTEM-1B / sul3 / tet(A) / sul2 / floR	iucA / iucB / iucC / iucD / iutA	1..114381	114381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T213631 WP_001312851.1 NZ_CP081887:c72421-72272 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T213631 NZ_CP109744:c1480437-1480159 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGCGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTAGCTCGCGCTAAGGGAATGGCGAACATTGCGGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACCCTTGCAGTG
ATGAAGGTACTCGGCATTGAATTGACACTTAAAAAATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT213631 NZ_CP081887:72465-72526 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin