Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3613504..3614183 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag K5O11_RS17340 Protein ID WP_000057541.1
Coordinates 3613881..3614183 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag K5O11_RS17335 Protein ID WP_000806442.1
Coordinates 3613504..3613845 (-) Length 114 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS17325 (3609747) 3609747..3610679 - 933 WP_000883039.1 glutaminase A -
K5O11_RS17330 (3610942) 3610942..3613446 + 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
K5O11_RS17335 (3613504) 3613504..3613845 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
K5O11_RS17340 (3613881) 3613881..3614183 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K5O11_RS17345 (3614316) 3614316..3615110 + 795 WP_000365157.1 TraB/GumN family protein -
K5O11_RS17350 (3615314) 3615314..3615793 + 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
K5O11_RS17355 (3615830) 3615830..3617482 - 1653 WP_000771723.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
K5O11_RS17360 (3617700) 3617700..3618920 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-98)

Antitoxin

(1-100)


Sequences


Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T213621 WP_000057541.1 NZ_CP081881:c3614183-3613881 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T213621 NZ_CP109739:2398463-2398885 [Serratia nevei]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACTCAGCTGTTTTCCCTGTTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTTTGGTCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGCGAGCTGCGGCTGTTGGCGGACCGGCGCTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGCTGACGCTGCAGCCGACGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGCCGG
TGGGTGACGGCGAGGAGGCCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT213621 WP_000806442.1 NZ_CP081881:c3613845-3613504 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT213621 NZ_CP109739:2398216-2398482 [Serratia nevei]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGCCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGTTGTTCATCCGCCTGCTGGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACAGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1


Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References