Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2385178..2385740 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag K5O11_RS11345 Protein ID WP_000605676.1
Coordinates 2385462..2385740 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K5O11_RS11340 Protein ID WP_000781370.1
Coordinates 2385178..2385462 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS11325 (2380537) 2380537..2383584 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
K5O11_RS11330 (2383597) 2383597..2384481 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
K5O11_RS11335 (2384474) 2384474..2385127 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
K5O11_RS11340 (2385178) 2385178..2385462 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K5O11_RS11345 (2385462) 2385462..2385740 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K5O11_RS11350 (2385926) 2385926..2386936 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
K5O11_RS11355 (2387070) 2387070..2388767 - 1698 WP_000433464.1 malate dehydrogenase -
K5O11_RS11360 (2388924) 2388924..2389061 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K5O11_RS11365 (2389163) 2389163..2389378 - 216 WP_000495766.1 biofilm-dependent modulation protein -
K5O11_RS11370 (2389723) 2389723..2390154 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T213611 WP_000605676.1 NZ_CP081881:c2385740-2385462 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T213611 NZ_CP109731:c78010-77525 [Enterobacter roggenkampii]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT213611 WP_000781370.1 NZ_CP081881:c2385462-2385178 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT213611 NZ_CP109731:c78264-77998 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References