Detailed information of TA system TA213604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 679053..679852
• Replicon: chromosome
• Accession: NZ_CP081881
• Organism: Escherichia coli strain WW2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: K5O11_RS03290
• Protein ID: WP_000347273.1
• Coordinates: 679053..679517 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: K5O11_RS03295
• Protein ID: WP_001308975.1
• Coordinates: 679517..679852 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O11_RS03260 (674054)	674054..674488	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
K5O11_RS03265 (674506)	674506..675384	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
K5O11_RS03270 (675374)	675374..676153	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
K5O11_RS03275 (676164)	676164..676637	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K5O11_RS03280 (676660)	676660..677940	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
K5O11_RS03285 (678189)	678189..678998	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
K5O11_RS03290 (679053)	679053..679517	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K5O11_RS03295 (679517)	679517..679852	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K5O11_RS03300 (680001)	680001..681572	-	1572	WP_001273756.1	galactarate dehydratase	-
K5O11_RS03305 (681947)	681947..683281	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
K5O11_RS03310 (683297)	683297..684067	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T213604 WP_000347273.1 NZ_CP081881:c679517-679053 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T213604 NZ_CP109727:c1672193-1671975 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACTAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT213604 WP_001308975.1 NZ_CP081881:c679852-679517 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT213604 NZ_CP109727:c1672595-1672221 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08