Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331161..331747 Replicon chromosome
Accession NZ_CP081865
Organism Klebsiella pneumoniae strain K5880C

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag K6J95_RS01525 Protein ID WP_002920800.1
Coordinates 331379..331747 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K6J95_RS01520 Protein ID WP_004174006.1
Coordinates 331161..331382 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J95_RS01500 (327318) 327318..328244 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K6J95_RS01505 (328241) 328241..329518 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K6J95_RS01510 (329515) 329515..330282 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K6J95_RS01515 (330284) 330284..330997 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K6J95_RS01520 (331161) 331161..331382 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K6J95_RS01525 (331379) 331379..331747 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K6J95_RS01530 (332020) 332020..333336 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K6J95_RS01535 (333443) 333443..334330 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K6J95_RS01540 (334327) 334327..335172 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K6J95_RS01545 (335174) 335174..336244 + 1071 WP_032433652.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 328241..336981 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T213574 WP_002920800.1 NZ_CP081865:331379-331747 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T213574 NZ_CP109692:2675076-2675447 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTTTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTTGGGCATCACCGGGGTCGTTTCACCTTACCAGCTGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT213574 WP_004174006.1 NZ_CP081865:331161-331382 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT213574 NZ_CP109692:2674831-2675073 [Enterobacter asburiae]
ATGATTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGAAGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References