Detailed information of TA system TA213547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57920..58674
• Replicon: chromosome
• Accession: NZ_CP081858
• Organism: Salmonella enterica subsp. enterica serovar Schwarzengrund strain S16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: K6B66_RS00275
• Protein ID: WP_000558166.1
• Coordinates: 58363..58674 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6B66_RS00270
• Protein ID: WP_001259009.1
• Coordinates: 57920..58366 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6B66_RS00240 (53096)	53096..53992	+	897	WP_001520529.1	sugar kinase	-
K6B66_RS00245 (54026)	54026..54829	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
K6B66_RS00250 (55020)	55020..55619	+	600	WP_000965695.1	glucose-1-phosphatase	-
K6B66_RS00255 (55613)	55613..56485	+	873	WP_000921423.1	virulence factor BrkB family protein	-
K6B66_RS00260 (56482)	56482..56919	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
K6B66_RS00265 (56964)	56964..57905	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K6B66_RS00270 (57920)	57920..58366	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
K6B66_RS00275 (58363)	58363..58674	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
K6B66_RS00280 (58760)	58760..59689	-	930	WP_001127691.1	alpha/beta hydrolase	-
K6B66_RS00285 (59907)	59907..60218	+	312	WP_001159631.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
K6B66_RS00290 (60219)	60219..60509	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
K6B66_RS00295 (60556)	60556..61485	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
K6B66_RS00300 (61482)	61482..62117	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K6B66_RS00305 (62114)	62114..63016	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T213547 WP_000558166.1 NZ_CP081858:c58674-58363 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T213547 NZ_CP109615:61065-61370 [Klebsiella sp. KP20-425-1]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT213547 WP_001259009.1 NZ_CP081858:c58366-57920 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT213547 NZ_CP109615:60845-61063 [Klebsiella sp. KP20-425-1]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90