Detailed information of TA system TA021351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2950955..2951682
• Replicon: chromosome
• Accession: NC_007613
• Organism: Shigella boydii Sb227

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q31WV0
• Locus tag: SBO_RS16365
• Protein ID: WP_000550185.1
• Coordinates: 2951368..2951682 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SBO_RS16360
• Protein ID: WP_000560271.1
• Coordinates: 2950955..2951371 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBO_RS29505	2946115..2947161	+	1047	WP_000695473.1	alpha-glucosidase	-
SBO_RS29510	2947627..2948466	+	840	Protein_2920	alpha-glucosidase	-
SBO_RS16355	2948892..2950910	+	2019	WP_000121441.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
SBO_RS16360	2950955..2951371	-	417	WP_000560271.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
SBO_RS16365	2951368..2951682	-	315	WP_000550185.1	type II toxin-antitoxin system toxin HigB	Toxin
SBO_RS16375	2951967..2953103	-	1137	WP_000018685.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
SBO_RS16380	2953188..2953691	+	504	WP_001295542.1	M48 family metallopeptidase	-
SBO_RS16385	2953768..2954451	+	684	WP_001183038.1	vancomycin high temperature exclusion protein	-
SBO_RS16390	2954530..2955516	+	987	WP_004986115.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12133.19 Da
• Isoelectric Point: 10.0409

>T21351 WP_000550185.1 NC_007613:c2951682-2951368 [Shigella boydii Sb227]
MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T21351 NC_007613:c2951682-2951368 [Shigella boydii Sb227]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACATGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15007.47 Da
• Isoelectric Point: 4.4547

>AT21351 WP_000560271.1 NC_007613:c2951371-2950955 [Shigella boydii Sb227]
MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT21351 NC_007613:c2951371-2950955 [Shigella boydii Sb227]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGTACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: Q31WV0