Detailed information of TA system TA213509

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 109796..110222
• Replicon: plasmid pF726925-1
• Accession: NZ_CP081821
• Organism: Klebsiella pneumoniae strain F726925

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K5O13_RS27605
• Protein ID: WP_001372321.1
• Coordinates: 109796..109921 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	109998..110222 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O13_RS27570 (104851)	104851..105078	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
K5O13_RS27575 (105172)	105172..105858	-	687	WP_015059009.1	PAS domain-containing protein	-
K5O13_RS27580 (106049)	106049..106432	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K5O13_RS27585 (106709)	106709..107356	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K5O13_RS27590 (107653)	107653..108474	-	822	WP_001234445.1	DUF932 domain-containing protein	-
K5O13_RS27595 (108585)	108585..108881	-	297	WP_001272251.1	hypothetical protein	-
K5O13_RS27600 (109181)	109181..109477	+	297	Protein_142	hypothetical protein	-
K5O13_RS27605 (109796)	109796..109921	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K5O13_RS28810 (109863)	109863..110012	-	150	Protein_144	plasmid maintenance protein Mok	-
- (109998)	109998..110222	-	225	NuclAT_0	-	Antitoxin
- (109998)	109998..110222	-	225	NuclAT_0	-	Antitoxin
- (109998)	109998..110222	-	225	NuclAT_0	-	Antitoxin
- (109998)	109998..110222	-	225	NuclAT_0	-	Antitoxin
K5O13_RS27610 (110234)	110234..110953	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
K5O13_RS27615 (110950)	110950..111384	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K5O13_RS27620 (111453)	111453..113476	-	2024	Protein_147	ParB/RepB/Spo0J family partition protein	-
K5O13_RS27625 (113537)	113537..113770	-	234	WP_000006003.1	DUF905 family protein	-
K5O13_RS27630 (113828)	113828..114355	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
K5O13_RS28815 (114657)	114657..115112	+	456	Protein_150	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2	-	1..172862	172862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T213509 WP_001372321.1 NZ_CP081821:c109921-109796 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T213509 NZ_CP109607:350588-350935 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT213509 NZ_CP081821:c110222-109998 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin