Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2815452..2816098 Replicon chromosome
Accession NZ_CP081816
Organism Klebsiella pneumoniae strain AR8416

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag K5O16_RS14365 Protein ID WP_016529833.1
Coordinates 2815452..2815799 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K5O16_RS14370 Protein ID WP_002920557.1
Coordinates 2815799..2816098 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O16_RS14355 2811378..2812811 + 1434 WP_002920564.1 glycogen synthase GlgA -
K5O16_RS14360 2812829..2815276 + 2448 WP_002920561.1 glycogen phosphorylase -
K5O16_RS14365 2815452..2815799 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K5O16_RS14370 2815799..2816098 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K5O16_RS14375 2816161..2817669 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
K5O16_RS14380 2817874..2818203 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K5O16_RS14385 2818254..2819084 + 831 WP_024622927.1 rhomboid family intramembrane serine protease GlpG -
K5O16_RS14390 2819134..2819892 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T213478 WP_016529833.1 NZ_CP081816:2815452-2815799 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T213478 NZ_CP109603:22419-22643 [Pseudomonas putida]
GTGCGCAGTCGTGAAGTAATTCAGATCATTGAGGCAGACGGGTGGTACGAGGTCGAGGTCAAGGGCAGTCATCATCAGTT
CAGGCACCCGGTAAAAAGAGGGAGAGTAACGGTGCCACATCCTAAAAGTGATCTGCCTAAGGGCACTGTACATAACATCT
TGAAGCAAGCTGGGCTGAAACAGCCCAGTAGTCCCAGCAGCTCAATCATCAGGGGAGACGCGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT213478 WP_002920557.1 NZ_CP081816:2815799-2816098 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT213478 NZ_CP109603:22643-23050 [Pseudomonas putida]
ATGAAGTTTCCGGTTGTATTGCACAAAGACGCCGATTCGGATTACGGCGTAACGGTTCCTGATGTGCCTGGCTGCTTTTC
AGCAGGCGCGAGTGTTTCCCAGGCGCTAGAGAATATTCAGGAAGCACTGGCATTGCATTTTGAAGGTCTGGTCGCTGATA
ACGAAGCGTTACCGCAGGCGCAAGAGATTGATGTTCATGTGGCTAACCCCGATTACCACGGCGGGGTCTGGGCAGTTGTG
GACTTCGATGTGACTCCGTATCTGGGCAAGGCGGTGCGGTTCAATGCAACGCTGCCGGAAAACCTGTTGCAACGGATCGA
TGAGAAGGTAAAGCGCGACCATCGGTATGCTTCTCGTTCCGGATTTCTGGCATCGGCAGCATTGCGAGAGTTGGCAAGCG
TGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References