Detailed information of TA system TA213470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4581732..4582334
• Replicon: chromosome
• Accession: NZ_CP081813
• Organism: Shigella flexneri strain WW1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: K5O10_RS21905
• Protein ID: WP_000897305.1
• Coordinates: 4582023..4582334 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5O10_RS21900
• Protein ID: WP_000356397.1
• Coordinates: 4581732..4582022 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O10_RS21875 (4577658)	4577658..4578560	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
K5O10_RS21880 (4578557)	4578557..4579192	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K5O10_RS21885 (4579189)	4579189..4580118	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
K5O10_RS21890 (4580448)	4580448..4580690	-	243	WP_001087409.1	protein YiiF	-
K5O10_RS21895 (4580909)	4580909..4581127	-	219	WP_001295676.1	CopG family transcriptional regulator	-
K5O10_RS21900 (4581732)	4581732..4582022	-	291	WP_000356397.1	NadS family protein	Antitoxin
K5O10_RS21905 (4582023)	4582023..4582334	-	312	WP_000897305.1	hypothetical protein	Toxin
K5O10_RS21910 (4582563)	4582563..4583471	+	909	WP_001299484.1	alpha/beta hydrolase	-
K5O10_RS21915 (4583535)	4583535..4584476	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
K5O10_RS21920 (4584521)	4584521..4584958	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
K5O10_RS21925 (4584955)	4584955..4585827	-	873	WP_000920762.1	virulence factor BrkB family protein	-
K5O10_RS21930 (4585821)	4585821..4586420	-	600	WP_001295269.1	glucose-1-phosphatase	-
K5O10_RS21935 (4586519)	4586519..4587304	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T213470 WP_000897305.1 NZ_CP081813:c4582334-4582023 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T213470 NZ_CP109601:3468548-3468766 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT213470 WP_000356397.1 NZ_CP081813:c4582022-4581732 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT213470 NZ_CP109601:3468148-3468522 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7