Detailed information of TA system TA213467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3753133..3753674
• Replicon: chromosome
• Accession: NZ_CP081813
• Organism: Shigella flexneri strain WW1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: U9XPG0
• Locus tag: K5O10_RS18100
• Protein ID: WP_000615976.1
• Coordinates: 3753396..3753674 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: K5O10_RS18095
• Protein ID: WP_112027186.1
• Coordinates: 3753133..3753393 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O10_RS18070 (3748169)	3748169..3749308	+	1140	WP_023155855.1	flagellar type III secretion system protein FlhB	-
K5O10_RS18075 (3749292)	3749292..3751385	+	2094	WP_001597626.1	flagellar biosynthesis protein FlhA	-
K5O10_RS18080 (3751490)	3751490..3751768	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
K5O10_RS18085 (3751761)	3751761..3752117	+	357	WP_001030483.1	type II toxin-antitoxin system HicB family antitoxin	-
K5O10_RS18090 (3752174)	3752174..3752947	-	774	WP_137472478.1	C40 family peptidase	-
K5O10_RS18095 (3753133)	3753133..3753393	+	261	WP_112027186.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
K5O10_RS18100 (3753396)	3753396..3753674	+	279	WP_000615976.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
K5O10_RS18105 (3753830)	3753830..3754570	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
K5O10_RS18110 (3754541)	3754541..3755308	-	768	WP_000333380.1	class II glutamine amidotransferase	-
K5O10_RS18115 (3755514)	3755514..3756092	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR	3751761..3769960	18199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10800.59 Da
• Isoelectric Point: 10.0702

>T213467 WP_000615976.1 NZ_CP081813:3753396-3753674 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T213467 NZ_CP109601:c2424824-2424648 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9400.78 Da
• Isoelectric Point: 5.6766

>AT213467 WP_112027186.1 NZ_CP081813:3753133-3753393 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNHLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT213467 NZ_CP109601:c2424602-2424186 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: U9XPG0