213451

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 583838..584637 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag K5O10_RS02845 Protein ID WP_000347266.1
Coordinates 583838..584302 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K5O10_RS02850 Protein ID WP_001307405.1
Coordinates 584302..584637 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS02815 (578839) 578839..579273 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K5O10_RS02820 (579291) 579291..580169 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
K5O10_RS02825 (580159) 580159..580938 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K5O10_RS02830 (580949) 580949..581422 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K5O10_RS02835 (581445) 581445..582725 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
K5O10_RS02840 (582974) 582974..583783 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K5O10_RS02845 (583838) 583838..584302 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K5O10_RS02850 (584302) 584302..584637 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K5O10_RS02855 (584786) 584786..586357 - 1572 WP_001273741.1 galactarate dehydratase -
K5O10_RS02860 (586732) 586732..588066 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K5O10_RS02865 (588082) 588082..588852 + 771 WP_001058232.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T213451 WP_000347266.1 NZ_CP081813:c584302-583838 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T213451 NZ_CP107722:c118806-118426 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT213451 WP_001307405.1 NZ_CP081813:c584637-584302 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT213451 NZ_CP107722:c119027-118806 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGGGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References