Detailed information of TA system TA213447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3828565..3829259
• Replicon: chromosome
• Accession: NZ_CP081791
• Organism: Escherichia coli strain CT01

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: J6O14_RS19140
• Protein ID: WP_001263493.1
• Coordinates: 3828565..3828963 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: J6O14_RS19145
• Protein ID: WP_001399937.1
• Coordinates: 3828966..3829259 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3824152)	3824152..3824232	-	81	NuclAT_12	-	-
- (3824152)	3824152..3824232	-	81	NuclAT_12	-	-
- (3824152)	3824152..3824232	-	81	NuclAT_12	-	-
- (3824152)	3824152..3824232	-	81	NuclAT_12	-	-
J6O14_RS19115 (3824828)	3824828..3825286	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J6O14_RS19120 (3825547)	3825547..3827004	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
J6O14_RS19125 (3827061)	3827061..3827582	-	522	Protein_3741	peptide chain release factor H	-
J6O14_RS19130 (3827578)	3827578..3827784	-	207	Protein_3742	RtcB family protein	-
J6O14_RS19135 (3828103)	3828103..3828555	-	453	WP_072146325.1	GNAT family N-acetyltransferase	-
J6O14_RS19140 (3828565)	3828565..3828963	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J6O14_RS19145 (3828966)	3828966..3829259	-	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J6O14_RS19150 (3829311)	3829311..3830366	-	1056	WP_001226161.1	DNA polymerase IV	-
J6O14_RS19155 (3830437)	3830437..3831222	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
J6O14_RS19160 (3831194)	3831194..3832906	+	1713	Protein_3748	flagellar biosynthesis protein FlhA	-
J6O14_RS19165 (3833224)	3833224..3833721	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3759099..3836425	77326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T213447 WP_001263493.1 NZ_CP081791:c3828963-3828565 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T213447 NZ_CP107720:c4397138-4396788 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT213447 WP_001399937.1 NZ_CP081791:c3829259-3828966 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT213447 NZ_CP107720:c4397383-4397132 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: S1EXB8