Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 23070..23797 | Replicon | plasmid unnamed 3 |
| Accession | NZ_CP081785 | ||
| Organism | Escherichia coli strain 0H26 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A663AUB1 |
| Locus tag | J6O44_RS26790 | Protein ID | WP_000558568.1 |
| Coordinates | 23070..23381 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | J6O44_RS26795 | Protein ID | WP_000990392.1 |
| Coordinates | 23378..23797 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J6O44_RS26755 (J6O44_26630) | 18256..18567 | + | 312 | WP_024191312.1 | hypothetical protein | - |
| J6O44_RS26760 (J6O44_26635) | 18657..19742 | - | 1086 | WP_001148851.1 | iron-containing alcohol dehydrogenase family protein | - |
| J6O44_RS26765 (J6O44_26640) | 19776..20933 | - | 1158 | WP_000217345.1 | alpha-hydroxy acid oxidase | - |
| J6O44_RS26770 (J6O44_26645) | 20930..21211 | - | 282 | WP_223596855.1 | aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme | - |
| J6O44_RS26775 (J6O44_26650) | 21251..21955 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| J6O44_RS26780 (J6O44_26655) | 22019..22429 | + | 411 | Protein_26 | lactate utilization protein C | - |
| J6O44_RS26785 (J6O44_26660) | 22479..22859 | - | 381 | Protein_27 | gluconate permease | - |
| J6O44_RS26790 (J6O44_26665) | 23070..23381 | + | 312 | WP_000558568.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| J6O44_RS26795 (J6O44_26670) | 23378..23797 | + | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| J6O44_RS26805 | 25241..25441 | - | 201 | Protein_31 | hypothetical protein | - |
| J6O44_RS26810 (J6O44_26685) | 25603..25953 | + | 351 | WP_000928911.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| J6O44_RS26815 (J6O44_26690) | 26258..26734 | - | 477 | WP_000043177.1 | Dps family protein | - |
| J6O44_RS26820 (J6O44_26695) | 26858..27805 | - | 948 | Protein_34 | transposase | - |
| J6O44_RS26825 (J6O44_26700) | 27871..28575 | + | 705 | WP_088765929.1 | IS6-like element IS26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..69246 | 69246 | |
| - | inside | IScluster/Tn | - | - | 3677..36807 | 33130 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12372.11 Da Isoelectric Point: 10.0935
>T213418 WP_000558568.1 NZ_CP081785:23070-23381 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
>T213418 NZ_CP107616:465041-465430 [Acinetobacter baumannii]
TTGATCAACCATTCGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCGGAGTGGAAGAAGCTTAAAACTTTAGAAAAACTCCATTAA
TTGATCAACCATTCGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCGGAGTGGAAGAAGCTTAAAACTTTAGAAAAACTCCATTAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT213418 WP_000990392.1 NZ_CP081785:23378-23797 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
>AT213418 NZ_CP107616:464777-465034 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTTGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTTGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A663AUB1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |