Detailed information of TA system TA213394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1117929..1118656
• Replicon: chromosome
• Accession: NZ_CP081782
• Organism: Escherichia coli strain 0H26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J6O44_RS05470
• Protein ID: WP_000547564.1
• Coordinates: 1117929..1118240 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6O44_RS05475
• Protein ID: WP_000126294.1
• Coordinates: 1118237..1118656 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O44_RS05440 (1113071)	1113071..1114780	+	1710	WP_001288140.1	formate hydrogenlyase subunit HycE	-
J6O44_RS05445 (1114790)	1114790..1115332	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J6O44_RS05450 (1115332)	1115332..1116099	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
J6O44_RS05455 (1116096)	1116096..1116506	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J6O44_RS05460 (1116499)	1116499..1116969	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J6O44_RS05465 (1116994)	1116994..1117755	+	762	WP_001026446.1	hypothetical protein	-
J6O44_RS05470 (1117929)	1117929..1118240	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6O44_RS05475 (1118237)	1118237..1118656	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J6O44_RS05480 (1118770)	1118770..1120194	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J6O44_RS05485 (1120203)	1120203..1121660	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J6O44_RS05490 (1121920)	1121920..1122930	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
J6O44_RS05495 (1123079)	1123079..1123606	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T213394 WP_000547564.1 NZ_CP081782:1117929-1118240 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T213394 NZ_CP107599:464933-465322 [Acinetobacter baumannii]
TTGATCAACCATTCGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCGGAGTGGAAGAAGCTTAAAACTTTAGAAAAACTCCATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT213394 WP_000126294.1 NZ_CP081782:1118237-1118656 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT213394 NZ_CP107599:464669-464926 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTTGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5