Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 58808..59535 Replicon plasmid unnamed4
Accession NZ_CP081770
Organism Escherichia coli strain 0H18

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O59_RS26880 Protein ID WP_000558568.1
Coordinates 59224..59535 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O59_RS26875 Protein ID WP_000990392.1
Coordinates 58808..59227 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O59_RS26845 (J6O59_26680) 54031..54735 - 705 WP_088765929.1 IS6-like element IS26 family transposase -
J6O59_RS26850 (J6O59_26685) 54801..55747 + 947 Protein_40 transposase -
J6O59_RS26855 (J6O59_26690) 55871..56347 + 477 WP_000043177.1 Dps family protein -
J6O59_RS26860 (J6O59_26695) 56652..57002 - 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O59_RS26865 57164..57364 + 201 Protein_43 hypothetical protein -
J6O59_RS26870 (J6O59_26705) 57620..58740 + 1121 WP_088765928.1 IS3 family transposase -
J6O59_RS26875 (J6O59_26710) 58808..59227 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O59_RS26880 (J6O59_26715) 59224..59535 - 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O59_RS26885 (J6O59_26720) 59746..60126 + 381 Protein_47 gluconate permease -
J6O59_RS26890 (J6O59_26725) 60176..60586 - 411 Protein_48 lactate utilization protein C -
J6O59_RS26895 (J6O59_26730) 60650..61354 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O59_RS26900 (J6O59_26735) 61394..61675 + 282 WP_223596855.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
J6O59_RS26905 (J6O59_26740) 61672..62829 + 1158 WP_000217345.1 alpha-hydroxy acid oxidase -
J6O59_RS26910 (J6O59_26745) 62863..63948 + 1086 WP_001148851.1 iron-containing alcohol dehydrogenase family protein -
J6O59_RS26915 (J6O59_26750) 64038..64349 - 312 WP_024191312.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..69251 69251
- inside IScluster/Tn - - 45799..67990 22191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213360 WP_000558568.1 NZ_CP081770:c59535-59224 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213360 NZ_CP107576:2251228-2251509 [Pseudomonas asiatica]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGGATCGTTGTAGTGGTGGTATCGGTTGGCAAA
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213360 WP_000990392.1 NZ_CP081770:c59227-58808 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213360 NZ_CP107576:2250993-2251238 [Pseudomonas asiatica]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCCGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AGCGCTTGGAAGATCTCGAATTGAGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTTTCCCTGGATGAC
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References