Detailed information of TA system TA213336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1117927..1118654
• Replicon: chromosome
• Accession: NZ_CP081767
• Organism: Escherichia coli strain 0H18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J6O59_RS05480
• Protein ID: WP_000547564.1
• Coordinates: 1117927..1118238 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6O59_RS05485
• Protein ID: WP_000126294.1
• Coordinates: 1118235..1118654 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O59_RS05450 (1113069)	1113069..1114778	+	1710	WP_001288140.1	formate hydrogenlyase subunit HycE	-
J6O59_RS05455 (1114788)	1114788..1115330	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J6O59_RS05460 (1115330)	1115330..1116097	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
J6O59_RS05465 (1116094)	1116094..1116504	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J6O59_RS05470 (1116497)	1116497..1116967	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J6O59_RS05475 (1116992)	1116992..1117753	+	762	WP_001026446.1	hypothetical protein	-
J6O59_RS05480 (1117927)	1117927..1118238	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6O59_RS05485 (1118235)	1118235..1118654	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J6O59_RS05490 (1118768)	1118768..1120192	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J6O59_RS05495 (1120201)	1120201..1121658	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J6O59_RS05500 (1121918)	1121918..1122928	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
J6O59_RS05505 (1123077)	1123077..1123604	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T213336 WP_000547564.1 NZ_CP081767:1117927-1118238 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T213336 NZ_CP107556:489473-489823 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAACGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT213336 WP_000126294.1 NZ_CP081767:1118235-1118654 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT213336 NZ_CP107556:489186-489467 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5