Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 708206..708933 Replicon chromosome
Accession NZ_CP081767
Organism Escherichia coli strain 0H18

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag J6O59_RS03515 Protein ID WP_000550189.1
Coordinates 708206..708520 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J6O59_RS03520 Protein ID WP_000560266.1
Coordinates 708517..708933 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O59_RS03495 (704371) 704371..705357 - 987 WP_223584379.1 Gfo/Idh/MocA family oxidoreductase -
J6O59_RS03500 (705436) 705436..706119 - 684 WP_001183040.1 vancomycin high temperature exclusion protein -
J6O59_RS03505 (706196) 706196..706699 - 504 WP_001295542.1 M48 family metallopeptidase -
J6O59_RS03510 (706785) 706785..707921 + 1137 WP_000018697.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
J6O59_RS03515 (708206) 708206..708520 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
J6O59_RS03520 (708517) 708517..708933 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J6O59_RS03525 (708978) 708978..710996 - 2019 WP_000121421.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
J6O59_RS03530 (711522) 711522..713873 - 2352 WP_000695498.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T213332 WP_000550189.1 NZ_CP081767:708206-708520 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T213332 NZ_CP107548:1709685-1710110 [Pusillimonas sp. M17]
ATGATTCTGCTGGATACAAACGTTATATCGGAGCAATTTCGAGCCGTACCCGATGAATCGGTGATTGACTGGTTGAATCG
TCAGCCGCTGGAAACGCTTTATCTGGCATCCATGACCGTGGCTGAACTGCGGGCCTGTGTTGCCCTGATGCCCGCAGGTA
AGCGCCGGACAGTGCTCAGTGACAACATCGAACACCAGGTGTTGCCGGTATTTGTGGGCCGGGTGCTGTCGTTTGATATG
GCTTGCACACGCGCCTATGCCGATGTGCTGGCAGCCGCCCGCAAGTCTGGCAGCGGCATCGAAGCGGCTGATGCCATCAT
CGCGGCGATTGCTCTGGATAACGGTTTCAGCGTGGCGACAAGGGACGTAAGCCCGTTCCTGGCGGCCGGCGTGAAAGTCA
TCAGTCCTTGGGAAGATCAAGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT213332 WP_000560266.1 NZ_CP081767:708517-708933 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT213332 NZ_CP107548:1709440-1709688 [Pusillimonas sp. M17]
ATGAGAGCAGTAACGATCAGGAATGTGCCGGAAGAGGTGCATCGTGCCATCCGGGTCAGGGCGGCGCAGAACGGCCGTAC
ATTGCAAGCCGAGATGTGCGAAATATTGGCTACCGCCGTGAAACCGGAAGGCCGGGTAAAGCTGGGCGACCTGCTGGCAG
ACATCGGGCGCAAGGTGAAATTGACCGACGAAGAAATGGCGGTTTTTGACCGTGACCATTCACCCGCCCGCGCAGCGAGT
TTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References