Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 115982..116709 Replicon plasmid unnamed1
Accession NZ_CP081763
Organism Escherichia coli strain CX21

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O46_RS24985 Protein ID WP_000558568.1
Coordinates 116398..116709 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O46_RS24980 Protein ID WP_000990392.1
Coordinates 115982..116401 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O46_RS24950 (J6O46_24850) 111204..111908 - 705 WP_088765929.1 IS6-like element IS26 family transposase -
J6O46_RS24955 (J6O46_24855) 111974..112921 + 948 Protein_96 transposase -
J6O46_RS24960 (J6O46_24860) 113045..113521 + 477 WP_000043177.1 Dps family protein -
J6O46_RS24965 (J6O46_24865) 113826..114176 - 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O46_RS24970 114338..114538 + 201 Protein_99 hypothetical protein -
J6O46_RS24975 (J6O46_24875) 114794..115914 + 1121 WP_088765928.1 IS3 family transposase -
J6O46_RS24980 (J6O46_24880) 115982..116401 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O46_RS24985 (J6O46_24885) 116398..116709 - 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O46_RS24990 (J6O46_24890) 116920..117300 + 381 Protein_103 gluconate permease -
J6O46_RS24995 (J6O46_24895) 117350..117811 - 462 Protein_104 lactate utilization protein C -
J6O46_RS25000 (J6O46_24900) 117876..118580 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O46_RS25005 (J6O46_24905) 118633..119490 + 858 Protein_106 APC family permease -
J6O46_RS25010 (J6O46_24910) 119598..120680 - 1083 WP_032140505.1 lytic murein transglycosylase B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146661 146661
- inside IScluster/Tn - - 109861..121193 11332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213327 WP_000558568.1 NZ_CP081763:c116709-116398 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213327 NZ_CP107548:c981873-981577 [Pusillimonas sp. M17]
ATGATCGAGCTAAAGCAGACAAAAACCTACGAGAAGTGGGAAGCCAGGCTACGTGACAAACGAGCAAAAACCATCATCGC
AGCGCGTTTGATGCGATTGGCGGAAGGTTTGCCAGGCGATATTGAATCGGTAGGCGAAGGAGTTAACGAATTGCGGATTC
ACTACGGCCCGGGCTATCGCGTCTACTTCCAGCGACGGGGAAACCTTCTGGTCGTATTGCTGTGCGGCGGAGATAAAAGC
ACTCAGGCCCGCGACATTGCCGCCGCAAAGAAACTGGCCAAAGAATGGAGTGAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213327 WP_000990392.1 NZ_CP081763:c116401-115982 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213327 NZ_CP107548:c981575-981282 [Pusillimonas sp. M17]
ATGACTGAAAAACTGACAACCTACGACCCTGCCACCGCCCTGGTCGATGATGAAGAAATCGCATTCTTCATGGCTGATGC
CTTTGAAACAGGCGATGCAGCCTATATTGCCAAGGCTCTTGGCGTGGTTGCTCGCGCCAAAGGGATGACAGAAATAGCAA
AAAAAACCGGACTTTCCCGTGAGCAGCTTTATCGGTCGTTCAGTGAGCGTGGCAATCCTACTTTGAAGACTACTCTAGCC
GTGATGCGAGCGCTTGGCGTCGATATGACGGCCAAGCCGCATGTCGCGAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References