Detailed information of TA system TA213307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1164034..1164761
• Replicon: chromosome
• Accession: NZ_CP081762
• Organism: Escherichia coli strain CX21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J6O46_RS05690
• Protein ID: WP_000547564.1
• Coordinates: 1164034..1164345 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6O46_RS05695
• Protein ID: WP_000126294.1
• Coordinates: 1164342..1164761 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O46_RS05660 (1159176)	1159176..1160885	+	1710	WP_001288140.1	formate hydrogenlyase subunit HycE	-
J6O46_RS05665 (1160895)	1160895..1161437	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J6O46_RS05670 (1161437)	1161437..1162204	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
J6O46_RS05675 (1162201)	1162201..1162611	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J6O46_RS05680 (1162604)	1162604..1163074	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J6O46_RS05685 (1163099)	1163099..1163860	+	762	WP_001026446.1	hypothetical protein	-
J6O46_RS05690 (1164034)	1164034..1164345	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6O46_RS05695 (1164342)	1164342..1164761	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J6O46_RS05700 (1164875)	1164875..1166299	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J6O46_RS05705 (1166308)	1166308..1167765	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J6O46_RS05710 (1168025)	1168025..1169035	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
J6O46_RS05715 (1169184)	1169184..1169711	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1118518..1165195	46677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T213307 WP_000547564.1 NZ_CP081762:1164034-1164345 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T213307 NZ_CP107518:3608923-3609330 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT213307 WP_000126294.1 NZ_CP081762:1164342-1164761 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT213307 NZ_CP107518:3608676-3608942 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5