Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 29944..30671 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP081743 | ||
| Organism | Escherichia coli strain CX15 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A663AUB1 |
| Locus tag | J6O52_RS24430 | Protein ID | WP_000558568.1 |
| Coordinates | 29944..30255 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | J6O52_RS24435 | Protein ID | WP_000990392.1 |
| Coordinates | 30252..30671 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J6O52_RS24400 (J6O52_24320) | 25266..25963 | + | 698 | WP_223335778.1 | IS1 family transposase | - |
| J6O52_RS24405 (J6O52_24325) | 25973..27055 | + | 1083 | WP_032140505.1 | lytic murein transglycosylase B | - |
| J6O52_RS24410 (J6O52_24330) | 27163..28020 | - | 858 | Protein_31 | APC family permease | - |
| J6O52_RS24415 (J6O52_24335) | 28073..28777 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| J6O52_RS24420 (J6O52_24340) | 28842..29303 | + | 462 | Protein_33 | lactate utilization protein C | - |
| J6O52_RS24425 (J6O52_24345) | 29353..29733 | - | 381 | Protein_34 | gluconate permease | - |
| J6O52_RS24430 (J6O52_24350) | 29944..30255 | + | 312 | WP_000558568.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| J6O52_RS24435 (J6O52_24355) | 30252..30671 | + | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| J6O52_RS24445 | 32115..32315 | - | 201 | Protein_38 | hypothetical protein | - |
| J6O52_RS24450 (J6O52_24370) | 32477..32827 | + | 351 | WP_000928911.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| J6O52_RS24455 (J6O52_24375) | 33132..33608 | - | 477 | WP_000043177.1 | Dps family protein | - |
| J6O52_RS24460 (J6O52_24380) | 33732..34679 | - | 948 | Protein_41 | transposase | - |
| J6O52_RS24465 (J6O52_24385) | 34745..35449 | + | 705 | WP_088765929.1 | IS6-like element IS26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..146653 | 146653 | |
| - | inside | IScluster/Tn | - | - | 25266..36792 | 11526 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12372.11 Da Isoelectric Point: 10.0935
>T213294 WP_000558568.1 NZ_CP081743:29944-30255 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
>T213294 NZ_CP107514:c150760-150269 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT213294 WP_000990392.1 NZ_CP081743:30252-30671 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
>AT213294 NZ_CP107514:c151014-150748 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A663AUB1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |