Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 29944..30671 Replicon plasmid unnamed1
Accession NZ_CP081743
Organism Escherichia coli strain CX15

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O52_RS24430 Protein ID WP_000558568.1
Coordinates 29944..30255 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O52_RS24435 Protein ID WP_000990392.1
Coordinates 30252..30671 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O52_RS24400 (J6O52_24320) 25266..25963 + 698 WP_223335778.1 IS1 family transposase -
J6O52_RS24405 (J6O52_24325) 25973..27055 + 1083 WP_032140505.1 lytic murein transglycosylase B -
J6O52_RS24410 (J6O52_24330) 27163..28020 - 858 Protein_31 APC family permease -
J6O52_RS24415 (J6O52_24335) 28073..28777 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O52_RS24420 (J6O52_24340) 28842..29303 + 462 Protein_33 lactate utilization protein C -
J6O52_RS24425 (J6O52_24345) 29353..29733 - 381 Protein_34 gluconate permease -
J6O52_RS24430 (J6O52_24350) 29944..30255 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O52_RS24435 (J6O52_24355) 30252..30671 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O52_RS24445 32115..32315 - 201 Protein_38 hypothetical protein -
J6O52_RS24450 (J6O52_24370) 32477..32827 + 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O52_RS24455 (J6O52_24375) 33132..33608 - 477 WP_000043177.1 Dps family protein -
J6O52_RS24460 (J6O52_24380) 33732..34679 - 948 Protein_41 transposase -
J6O52_RS24465 (J6O52_24385) 34745..35449 + 705 WP_088765929.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146653 146653
- inside IScluster/Tn - - 25266..36792 11526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213294 WP_000558568.1 NZ_CP081743:29944-30255 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213294 NZ_CP107514:c150760-150269 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213294 WP_000990392.1 NZ_CP081743:30252-30671 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213294 NZ_CP107514:c151014-150748 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References