Detailed information of TA system TA213276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1961248..1961750
• Replicon: chromosome
• Accession: NZ_CP081742
• Organism: Escherichia coli strain CX15

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: J6O52_RS09440
• Protein ID: WP_000767829.1
• Coordinates: 1961496..1961750 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: J6O52_RS09435
• Protein ID: WP_001259255.1
• Coordinates: 1961248..1961499 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O52_RS09410 (1956426)	1956426..1957493	-	1068	WP_000080142.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
J6O52_RS09415 (1957493)	1957493..1958563	-	1071	WP_032248775.1	histidinol-phosphate transaminase	-
J6O52_RS09420 (1958560)	1958560..1959864	-	1305	WP_016240067.1	histidinol dehydrogenase	-
J6O52_RS09425 (1959870)	1959870..1960769	-	900	WP_000131786.1	ATP phosphoribosyltransferase	-
J6O52_RS09430 (1960915)	1960915..1960965	-	51	WP_001364200.1	his operon leader peptide	-
J6O52_RS09435 (1961248)	1961248..1961499	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
J6O52_RS09440 (1961496)	1961496..1961750	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
J6O52_RS09445 (1961833)	1961833..1962657	+	825	WP_000754749.1	SDR family oxidoreductase	-
J6O52_RS09450 (1962703)	1962703..1963632	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
J6O52_RS09455 (1963847)	1963847..1963909	+	63	WP_010723108.1	membrane protein YoeI	-
J6O52_RS09460 (1963899)	1963899..1965257	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
J6O52_RS09465 (1965436)	1965436..1966494	+	1059	WP_000492297.1	thiosulfate utilization transporter TsuA/YeeE	-
J6O52_RS09470 (1966508)	1966508..1966735	+	228	WP_000985279.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T213276 WP_000767829.1 NZ_CP081742:1961496-1961750 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T213276 NZ_CP107509:c125831-125415 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT213276 WP_001259255.1 NZ_CP081742:1961248-1961499 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT213276 NZ_CP107509:c126058-125828 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0