Detailed information of TA system TA213272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 819739..820432
• Replicon: chromosome
• Accession: NZ_CP081742
• Organism: Escherichia coli strain CX15

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: J6O52_RS04070
• Protein ID: WP_000415584.1
• Coordinates: 819739..820035 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: J6O52_RS04075
• Protein ID: WP_000650107.1
• Coordinates: 820037..820432 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O52_RS04035 (814873)	814873..815352	+	480	WP_000065333.1	Hcp family type VI secretion system effector	-
J6O52_RS04040 (815355)	815355..816065	+	711	WP_000834029.1	hypothetical protein	-
J6O52_RS04045 (816072)	816072..816404	+	333	WP_000914691.1	DUF2645 family protein	-
J6O52_RS04050 (816450)	816450..817799	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
J6O52_RS04055 (817796)	817796..818455	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
J6O52_RS04060 (818607)	818607..818999	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
J6O52_RS04065 (819052)	819052..819534	+	483	WP_000609258.1	GyrI-like domain-containing protein	-
J6O52_RS04070 (819739)	819739..820035	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
J6O52_RS04075 (820037)	820037..820432	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
J6O52_RS04080 (820565)	820565..822172	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
J6O52_RS04085 (822310)	822310..824568	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T213272 WP_000415584.1 NZ_CP081742:819739-820035 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T213272 NZ_CP107508:c4904502-4904218 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT213272 WP_000650107.1 NZ_CP081742:820037-820432 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT213272 NZ_CP107508:c4904734-4904492 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65