Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2215491..2216118 Replicon chromosome
Accession NC_007606
Organism Shigella dysenteriae Sd197

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag SDY_RS11845 Protein ID WP_024258354.1
Coordinates 2215491..2215820 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag SDY_RS11850 Protein ID WP_000147081.1
Coordinates 2215810..2216118 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SDY_RS11810 2211162..2211482 + 321 Protein_2287 ABC transporter substrate-binding protein ArtJ -
SDY_RS11815 2211498..2211761 - 264 Protein_2288 IS1 family transposase -
SDY_RS11825 2213028..2213461 - 434 Protein_2290 IS1-like element IS1N family transposase -
SDY_RS11830 2213518..2213658 - 141 Protein_2291 HNH endonuclease -
SDY_RS11835 2213696..2214418 - 723 WP_001004003.1 DNA-binding protein -
SDY_RS11840 2214493..2215170 - 678 WP_000957540.1 phage antirepressor Ant -
SDY_RS11845 2215491..2215820 + 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SDY_RS11850 2215810..2216118 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
SDY_RS11855 2216115..2216336 - 222 WP_001254231.1 NinE family protein -
SDY_RS11860 2216333..2216743 - 411 WP_000814621.1 recombination protein NinB -
SDY_RS11870 2218161..2219165 + 1005 Protein_2299 tyrosine-type recombinase/integrase -
SDY_RS11875 2219223..2219783 - 561 WP_001215754.1 DUF1175 domain-containing protein -
SDY_RS11880 2219780..2220121 - 342 Protein_2301 DUF2138 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2201259..2253854 52595
- inside IScluster/Tn - - 2204333..2218027 13694


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T21327 WP_024258354.1 NC_007606:2215491-2215820 [Shigella dysenteriae Sd197]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T21327 NC_007606:2215491-2215820 [Shigella dysenteriae Sd197]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT21327 WP_000147081.1 NC_007606:2215810-2216118 [Shigella dysenteriae Sd197]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT21327 NC_007606:2215810-2216118 [Shigella dysenteriae Sd197]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References